Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100315
• Name: oriT_p5217
• Organism: Escherichia coli
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_011799 (1876..1957 [-], 82 nt)
• oriT length: 82 nt
• IRs (inverted repeats): 3..12, 16..25 (GTGTCGGGGC..GCCATGACCC)
• Location of nic site: 56..57
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 82 nt

>oriT_p5217
GGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGGCATCAGTGCGGAT

oriT secondary structure

Predicted by RNAfold.

Relaxase

MobA_p5217

• ID: 386
• GenBank: YP_002455781
• Name: MobA_p5217
• UniProt ID: B8QJ14
• Length: 517 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 517 a.a.
• Molecular weight: 57739.13 Da
• Isoelectric Point: 6.3953

>YP_002455781.1 plasmid mobilization protein MobA (plasmid) [Escherichia coli]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRILTLPDNLPETKQALAESVTRGIDALYHVGEIKGRQDVIQALTEAGLEVVRVTRT
SISIADPNGGKNIRLKGAFYEQSFTDGRGVREKAERESRIYRDNAERRVQQARKICKQGCDIKRDENQRR
YSPVHSFDRGITEKTPGRGERGDDAAQEGRVKAGREYGHDVTGDGHFPVYREWRDALVSWRADTGEPGRN
QDTGRNIAETEREDMGRGVCAGREKEISGHPAGEIGNGDSLSGEGLGTTDGVTQSDGAGKTFAERIRATA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQEIRDERLIQE
REHALSLERERQPEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (57-237)

Protein structure

• AlphaFold DB: B8QJ14

Host bacterium

• ID: 777
• GenBank: NC_011799
• Plasmid name: p5217
• Incompatibility group: ColRNAI
• Plasmid size: 5217 bp
• Coordinate of oriT [Strand]: 1876..1957 [-]
• Host baterium: Escherichia coli

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -