Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100311
Name   oriT_pEC278 in_silico
Organism   Escherichia coli
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_011418 (705..879 [-], 175 nt)
oriT length   175 nt
IRs (inverted repeats)      2..11, 15..24  (GTTTCGGGGC..GCCCTGAACC)
Location of nic site      55..56
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 175 nt

>oriT_pEC278
GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAATATGTTGGCACGGATGAGGGTGTCAGTGAAGTGCTTCATGTGGCAGGAGAAAAAAGGCGGCACCGGCGCGTCAGCAGAATATGTGATACAGGATATATTCCGCTTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   382 GenBank   YP_002296023
Name   MobA_pEC278 insolico UniProt ID   Q6PP42
Length   499 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 499 a.a.        Molecular weight: 55380.75 Da        Isoelectric Point: 8.2087

>YP_002296023.1 MobA-like protein (plasmid) [Escherichia coli]
MIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELIDASPYVKKYTSGVLSFAEADLPSGQR
EKLMASFERVLMPGLDKDQYSILWVEHRDKGRLELNFLIPNTELLTGRRLQPYYDRADRPRIDAWQTIVN
GRLGLHDPNAPENRRALVTPSALPKAKQEAAEAITRGLLALASSGELKTRQDVTEALESAGFEVVRTTKS
SISIADPDGGRNIRLKGAIYEQSFNAGEGLRAEIESAAAEYRRDAESRIQRAREICKSGTERKREENQRR
HPRPRPETVLSHEPAHERHGAHGQPDVADHRPGLRAADRLERGHSVVAGAADTRQLHDHPGAEEHAGHAV
REEQRRAALDLRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDGAGKTVAERIRAATAGLLEKAGRVGE
RLRGMADDVWSYATGERSAERARHGLEQAGAEFERAAAPVVERLNAVELHREQERVVQHQKALELERSQR
QRTYNSPSL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(58-238)


  Protein structure


Source ID Structure
AlphaFold DB Q6PP42


Host bacterium

ID   773 GenBank   NC_011418
Plasmid name   pEC278 Incompatibility group   ColRNAI
Plasmid size   4669 bp Coordinate of oriT [Strand]   705..879 [-]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -