Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100310
Name   oriT_pSE11-5 in_silico
Organism   Escherichia coli SE11
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_011408 (4542..4716 [-], 175 nt)
oriT length   175 nt
IRs (inverted repeats)      2..11, 15..24  (GTTTCGGGGC..GCCCTGAACC)
Location of nic site      55..56
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 175 nt

>oriT_pSE11-5
GGTTTCGGGGCGAAGCCCTGAACCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTACTATGTTGGCATGGATGCGGAAATCAGTGAAGTGCTTCATGTGACAGGAGAAAAAAGGCGGCACAGGCGCGTCAGCAGAATATGCGATACAGGATATATTCCGCTTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   381 GenBank   WP_000635811
Name   Nuclease_pSE11-5 insolico UniProt ID   B6ICT2
Length   517 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 517 a.a.        Molecular weight: 57877.53 Da        Isoelectric Point: 6.5220

>WP_000635811.1 nuclease [Escherichia coli]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRLRIDAWQTLVN
HHYGLHDPNAPENRRTLILPDNLPETKQALAESVTRGLEVLYHAGEIKGRQDVIHALTEVGLEVVRVTRS
SISIADPNGGRNIRLKGAFYEQSFTDGRGVREKAERESRIYRENAERRIQQARRICKRGCDLKRDENQRR
YSPVYGLDGGIAEKTPGRGERGDDAAPEGHVKAGREFGHEVAGDSLSPVYREWRDALVSRREDTDEPGRN
QGTGRDIAETEREDMGCGVCSGRGKKISGHSARDVSRGDALSGEGLDTTDGVIRNDRAGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGTALERADRTLEPVIQRELEIREERLIQE
REHALSLERERQLEIQERTLDGPSLSL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-237)


  Protein structure


Source ID Structure
AlphaFold DB B6ICT2


Host bacterium

ID   772 GenBank   NC_011408
Plasmid name   pSE11-5 Incompatibility group   ColRNAI
Plasmid size   5366 bp Coordinate of oriT [Strand]   4542..4716 [-]
Host baterium   Escherichia coli SE11

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -