Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100309
• Name: oriT_pSE11-4
• Organism: Escherichia coli SE11
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_011407 (3815..3902 [+], 88 nt)
• oriT length: 88 nt
• IRs (inverted repeats): 8..17, 21..30 (GTTTCGGGGC..GCCCTGAACC)
• Location of nic site: 61..62
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 88 nt

>oriT_pSE11-4
TCAGGGGGTTTCGGGGCGAAGCCCTGAACCAGTTACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGGCATCAGTGCAGATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Nuclease_pSE11-4

• ID: 380
• GenBank: WP_000635810
• Name: Nuclease_pSE11-4
• UniProt ID: B6ICS5
• Length: 517 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 517 a.a.
• Molecular weight: 57951.53 Da
• Isoelectric Point: 6.7023

>WP_000635810.1 nuclease [Escherichia coli]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRTLTLPDNLPETKQALAESVTRGLEVLYHAGEIKGRQDVIQALTEVGLEVVRVTRS
SISIADPNGGRNIRLKGAFYEQSFTDGRGVREKAERESRIYRENAERRIQQARRICKRGCDLKRDENQRR
YSPVHNVDRGIAEKTPGRGERGDDAAPEGHVKAGREFGHEVAGDSLSPVYREWRDALVSRREDTDEPGRN
QGTGRDIAETEREDMGCGVCSGRGKKISGHSARDVSRGDALSGEGLDTTDGVIRNDRAGNTFTERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQRELEIREERLIHE
REHALSLERERQLEIQERTLDGPSLSL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (57-237)

Protein structure

• AlphaFold DB: B6ICS5

Host bacterium

• ID: 771
• GenBank: NC_011407
• Plasmid name: pSE11-4
• Incompatibility group: ColRNAI
• Plasmid size: 6929 bp
• Coordinate of oriT [Strand]: 3815..3902 [+]
• Host baterium: Escherichia coli SE11

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -