Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100308
Name   oriT_pEC01 in_silico
Organism   Enterobacter cloacae
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_011404 (43..217 [-], 175 nt)
oriT length   175 nt
IRs (inverted repeats)      2..11, 15..24  (GTTTCGGGGC..GCCCTGAACC)
Location of nic site      55..56
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 175 nt

>oriT_pEC01
GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGCACGAATGCGGATGTCAGTGAAGTGCTTCATGTGGAAGGAGAAAAAATACGGCACCGGCGCGTCAGCAGAATATGCGATACAGGATATATTCCGCTTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   379 GenBank   YP_002290990
Name   MobA_pEC01 insolico UniProt ID   Q762E0
Length   500 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 500 a.a.        Molecular weight: 55274.90 Da        Isoelectric Point: 9.8372

>YP_002290990.1 MobA (plasmid) [Enterobacter cloacae]
MIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELIDASPYVKKYTSGVLSFAEADLPPGQR
EKLMASFERVLMPGLDKDQYSILWVEHADKGRLELNFLIPNTELLTGRRLQPYYDRADRPRIDAWQTIVN
GRLGLHDPNAPENRRALVTPSGLPKTKQEAAEAITRGLLTLASSGELKSPSGMSLTLFEGAGFLRVVRTT
KSSIKHCRPGRGANIRLKGAIYEQSFNAGEGLRAAIESAAAEYRRDAESRIQRARAVCQSGTERKREENQ
RRHPRPRAEYERGDGEKPAERTADCRADMAHHPDGVSAADRHEREHSVVAGAADNRQLHASSSAGRYPGE
NDRPDVGSALSGEQRRPEVSYPAAGDANRSHPLRRDDMDPPETGVAENDRAGKAVAERIRAATAGLLAKA
GRMGERLRGMAEHVWAYATGERGAERARVGLEHAGAEFKRAAAPLVRELNATEFRQRENEYQKTLELERQ
PQKTYRGPTL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-175)


  Protein structure


Source ID Structure
AlphaFold DB Q762E0


Host bacterium

ID   770 GenBank   NC_011404
Plasmid name   pEC01 Incompatibility group   Col440II
Plasmid size   5002 bp Coordinate of oriT [Strand]   43..217 [-]
Host baterium   Enterobacter cloacae

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -