Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100306
Name   oriT_pCCK1900 in_silico
Organism   Pasteurella multocida
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_011378 (5663..5823 [-], 161 nt)
oriT length   161 nt
IRs (inverted repeats)      IR1: 3..12, 16..25  (CCAGTTTCTC..GAGAAACCGG)
  IR2: 40..45, 49..54  (CCCTAC..GTAGGG)
Location of nic site      33..34
Conserved sequence flanking the
  nic site    		  TAAGTGCG|CCCT
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 161 nt

>oriT_pCCK1900
GGCCAGTTTCTCGAAGAGAAACCGGTAAGTGCGCCCTCCCCCTACAAAGTAGGGTCGGGATTGCCGCCGCTGTGCCTCCATGATAGCCTACGAGACAGCACATTAACAATGGGGTGTCAAGATGGTTAAGGGGAGCAACAAGGCGGCGGATCGGCTGGCCA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   377 GenBank   YP_002286770
Name   Mob_pCCK1900 insolico UniProt ID   B6ET86
Length   709 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 709 a.a.        Molecular weight: 77951.91 Da        Isoelectric Point: 7.2077

>YP_002286770.1 mobilization protein A (plasmid) [Pasteurella multocida]
MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESGHMPEFVERPADYWDAADLYERANGR
LFKEVEFALPVELTLDQQKALASEFAQHLTGAERLPYTLAIHAGGGENPHCHLMISERINDGIERPAAQW
FKRYNGKTPEKGGAQKTEALKPKAWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGP
NVVEMEGRGIRTDRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTG
RRSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGVALEAMANRDA
GFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQM
MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGK
TATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDF
IAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGM
DRGGPDFSM

  Protein domains


Predicted by InterproScan.

RepB DNA-primase from phage plasmid (RepB_primase)

(410-591)

MobA/MobL family (MobA_MobL)

(37-217)


  Protein structure


Source ID Structure
AlphaFold DB B6ET86


Host bacterium

ID   768 GenBank   NC_011378
Plasmid name   pCCK1900 Incompatibility group   IncQ1
Plasmid size   10226 bp Coordinate of oriT [Strand]   5663..5823 [-]
Host baterium   Pasteurella multocida

Cargo genes

Drug resistance gene   sul2, aph(3'')-Ib, aph(6)-Id, floR
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -