Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100302 |
| Name | oriT_pSL476_91  |
| Organism | Salmonella enterica subsp. enterica serovar Heidelberg str. SL476 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_011081 (38935..39044 [-], 110 nt) |
| oriT length | 110 nt |
| IRs (inverted repeats) | IR1: 24..31, 35..42 (GTCGGGGC..GCCCTGAC) IR2: 49..65, 72..88 (GCAATTGTAATAGCGTC..GACGGTATTACAATTGC) |
| Location of nic site | 96..97 |
| Conserved sequence flanking the nic site |
CATCCTG|T |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 110 nt
>oriT_pSL476_91
TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTCAGG
TCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTCAGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 373 | GenBank | WP_001306778 |
| Name | NikB_pSL476_91 |
UniProt ID | _ |
| Length | 899 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 899 a.a. Molecular weight: 104010.41 Da Isoelectric Point: 7.4265
>WP_001306778.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 44404..81302
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SEHA_RS00275 (SeHA_A0075) | 42120..44411 | - | 2292 | WP_001289276 | F-type conjugative transfer protein TrbC | - |
| SEHA_RS00280 (SeHA_A0076) | 44404..45474 | - | 1071 | WP_000151582 | IncI1-type conjugal transfer protein TrbB | trbB |
| SEHA_RS00285 (SeHA_A0077) | 45493..46701 | - | 1209 | WP_000121273 | IncI1-type conjugal transfer protein TrbA | trbA |
| SEHA_RS00290 (SeHA_A0078) | 46993..47145 | + | 153 | WP_001331364 | Hok/Gef family protein | - |
| SEHA_RS00295 (SeHA_A0079) | 47217..47468 | - | 252 | WP_001291964 | hypothetical protein | - |
| SEHA_RS27150 | 47967..48062 | + | 96 | WP_001303310 | DinQ-like type I toxin DqlB | - |
| SEHA_RS26735 (SeHA_A0080) | 48127..48303 | - | 177 | WP_001054897 | hypothetical protein | - |
| SEHA_RS00305 (SeHA_A0082) | 48695..48904 | + | 210 | WP_000062603 | HEAT repeat domain-containing protein | - |
| SEHA_RS00310 (SeHA_A0083) | 48976..49638 | - | 663 | WP_000644796 | plasmid IncI1-type surface exclusion protein ExcA | - |
| SEHA_RS00315 (SeHA_A0084) | 49709..51877 | - | 2169 | WP_000698354 | DotA/TraY family protein | traY |
| SEHA_RS00320 (SeHA_A0085) | 51974..52558 | - | 585 | WP_001037987 | IncI1-type conjugal transfer protein TraX | - |
| SEHA_RS00325 (SeHA_A0086) | 52587..53789 | - | 1203 | WP_001385654 | IncI1-type conjugal transfer protein TraW | traW |
| SEHA_RS00330 (SeHA_A0087) | 53756..54370 | - | 615 | WP_000337399 | IncI1-type conjugal transfer protein TraV | traV |
| SEHA_RS00335 (SeHA_A0088) | 54370..57414 | - | 3045 | WP_001024780 | IncI1-type conjugal transfer protein TraU | traU |
| SEHA_RS00340 (SeHA_A0089) | 57504..58304 | - | 801 | WP_001164788 | IncI1-type conjugal transfer protein TraT | traT |
| SEHA_RS00345 (SeHA_A0090) | 58288..58476 | - | 189 | WP_001277255 | putative conjugal transfer protein TraS | - |
| SEHA_RS00350 (SeHA_A0091) | 58540..58944 | - | 405 | WP_000086958 | IncI1-type conjugal transfer protein TraR | traR |
| SEHA_RS00355 (SeHA_A0092) | 58995..59522 | - | 528 | WP_001055569 | conjugal transfer protein TraQ | traQ |
| SEHA_RS00360 (SeHA_A0093) | 59522..60226 | - | 705 | WP_000801920 | IncI1-type conjugal transfer protein TraP | traP |
| SEHA_RS00365 (SeHA_A0094) | 60226..61515 | - | 1290 | WP_001272002 | conjugal transfer protein TraO | traO |
| SEHA_RS00370 (SeHA_A0095) | 61518..62501 | - | 984 | WP_001191877 | IncI1-type conjugal transfer protein TraN | traN |
| SEHA_RS00375 (SeHA_A0096) | 62512..63204 | - | 693 | WP_000138550 | DotI/IcmL family type IV secretion protein | traM |
| SEHA_RS00380 (SeHA_A0097) | 63201..63548 | - | 348 | WP_001055900 | conjugal transfer protein | traL |
| SEHA_RS00385 (SeHA_A0098) | 63566..67333 | - | 3768 | WP_001141531 | LPD7 domain-containing protein | - |
| SEHA_RS00390 (SeHA_A0099) | 67423..67974 | - | 552 | WP_000014584 | phospholipase D family protein | - |
| SEHA_RS24435 (SeHA_A0100) | 67989..68279 | - | 291 | WP_001299214 | hypothetical protein | traK |
| SEHA_RS00395 (SeHA_A0101) | 68276..69424 | - | 1149 | WP_001024972 | plasmid transfer ATPase TraJ | virB11 |
| SEHA_RS00400 (SeHA_A0102) | 69421..70239 | - | 819 | WP_000646097 | IncI1-type conjugal transfer lipoprotein TraI | traI |
| SEHA_RS00405 (SeHA_A0103) | 70236..70694 | - | 459 | WP_001079808 | IncI1-type conjugal transfer lipoprotein TraH | - |
| SEHA_RS00410 (SeHA_A0105) | 71089..71673 | - | 585 | WP_000977522 | histidine phosphatase family protein | - |
| SEHA_RS00415 (SeHA_A0106) | 71733..72935 | - | 1203 | WP_000976353 | conjugal transfer protein TraF | - |
| SEHA_RS00420 (SeHA_A0107) | 73021..73845 | - | 825 | WP_001238927 | conjugal transfer protein TraE | traE |
| SEHA_RS00425 (SeHA_A0108) | 73996..75150 | - | 1155 | WP_001139958 | site-specific integrase | - |
| SEHA_RS00435 (SeHA_A0109) | 75462..76796 | - | 1335 | WP_024132209 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| SEHA_RS24440 (SeHA_A0110) | 76796..77452 | - | 657 | WP_001193553 | prepilin peptidase | - |
| SEHA_RS00440 (SeHA_A0111) | 77437..77997 | - | 561 | WP_000014116 | lytic transglycosylase domain-containing protein | virB1 |
| SEHA_RS00445 (SeHA_A0112) | 78007..78621 | - | 615 | WP_000959785 | type 4 pilus major pilin | - |
| SEHA_RS00450 (SeHA_A0113) | 78639..79736 | - | 1098 | WP_001208805 | type II secretion system F family protein | - |
| SEHA_RS00455 (SeHA_A0114) | 79749..81302 | - | 1554 | WP_000362202 | ATPase, T2SS/T4P/T4SS family | virB11 |
| SEHA_RS00460 (SeHA_A0115) | 81313..81765 | - | 453 | WP_001247334 | type IV pilus biogenesis protein PilP | - |
| SEHA_RS00465 (SeHA_A0116) | 81752..83047 | - | 1296 | WP_000752772 | type 4b pilus protein PilO2 | - |
| SEHA_RS00470 (SeHA_A0117) | 83040..84722 | - | 1683 | WP_000748143 | PilN family type IVB pilus formation outer membrane protein | - |
| SEHA_RS00475 (SeHA_A0118) | 84736..85173 | - | 438 | WP_000539807 | type IV pilus biogenesis protein PilM | - |
| SEHA_RS24445 (SeHA_A0119) | 85173..86240 | - | 1068 | WP_001302629 | type IV pilus biogenesis lipoprotein PilL | - |
Host bacterium
| ID | 764 | GenBank | NC_011081 |
| Plasmid name | pSL476_91 | Incompatibility group | IncI1 |
| Plasmid size | 91374 bp | Coordinate of oriT [Strand] | 38935..39044 [-] |
| Host baterium | Salmonella enterica subsp. enterica serovar Heidelberg str. SL476 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |