Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100295
Name   oriT_pMAR7 in_silico
Organism   Escherichia coli
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_010862 (73269..73741 [+], 473 nt)
oriT length   473 nt
IRs (inverted repeats)      153..162, 165..174  (ATAGGGTCGT..ACGACCCTAT)
 242..249, 252..259  (GCAAAAAC..GTTTTTGC)
Location of nic site      268..269
Conserved sequence flanking the
  nic site    		  GTGGGGTGT|GG
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 473 nt

>oriT_pMAR7
AAGAAGAGCACCCCTAGCAGCGCCCCTAGGGGCATACTATAAAAAAACGCACGGCGCTGCTAGGGGCGGCCCTAATATATCCAAATGTTTTTCATGAAAATTGTCAGCACTGACCCTAACAAGGGTCGTCATAGGGTCGCTATAGGGTCGCTATAGGGTCGTCAACGACCCTATTTAATAATGTAAAATAAATTAAAATACATTATTTAGAACATAAACTAATGATTTAAAAAACAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCCATCTGCCTGATGTTTATAAATGAGATCTGCCATGCCACTGATTGCTTTGATCTTGCAGGCCGGGATTACAAAATAGATCCTGATTTACTGAGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   366 GenBank   YP_001965416
Name   Relaxase_pMAR7 insolico UniProt ID   Q0H0A7
Length   1763 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1763 a.a.        Molecular weight: 192804.75 Da        Isoelectric Point: 6.0281

>YP_001965416.1 oriT-specific relaxase; helicase (plasmid) [Escherichia coli]
MSKGYTFMMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGKLPDG
ADLSRMQDGSNKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSET
VLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREK
LKEQVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVR
MAEWMQTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLART
VGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSV
PRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLS
GELITGRRQLQEGMAFTPGNTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDA
GVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGV
LGRPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSNSLTLRDAQGETQVV
RISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVSDS
PFMALKLENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSF
TVVSEQIKARAGETLLETAISLQKTGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFTE
LGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQ
RAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTL
ASFLHDTQLLQRSGETPNFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFR
LQQTRSAADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQ
EAKLAEAQQKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGE
LGKEQVMVPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPRE
AVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQH
IDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQK
GTAHDVLEPKSDREVMNAERLFSTARELRDVVAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFD
RNGKSAGIWLNPLTTDDGNGLRGFSGEGREKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGV
VVRIAGEGRPWNPGTITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMA
ENKPDLPDGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDM
VRDLQKEKTLGGD

  Protein domains


Predicted by InterproScan.

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(582-633)

AAA domain (AAA_30)

(976-1163)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(640-719)

DNA helicase TraI (TraI_C)

(1441-1564)

TrwC relaxase (TrwC)

(17-290)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 47840..74137

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS433_RS00265 (MAR148) 47206..47433 + 228 WP_000450520 toxin-antitoxin system antitoxin VapB -
HS433_RS00270 (MAR111) 47433..47831 + 399 WP_000911313 type II toxin-antitoxin system VapC family toxin -
HS433_RS00275 (MAR048) 47840..49993 - 2154 WP_000009324 type IV conjugative transfer system coupling protein TraD virb4
HS433_RS00280 (MAR049) 50246..50977 - 732 WP_000850423 conjugal transfer complement resistance protein TraT -
HS433_RS00285 (MAR050) 50991..51500 - 510 WP_000628106 conjugal transfer entry exclusion protein TraS -
HS433_RS00290 (MAR051) 51497..54322 - 2826 WP_001007001 conjugal transfer mating-pair stabilization protein TraG traG
HS433_RS00295 (MAR052) 54319..55692 - 1374 WP_000488447 conjugal transfer pilus assembly protein TraH traH
HS433_RS00300 (MAR053) 55692..56072 - 381 WP_001411063 F-type conjugal transfer protein TrbF -
HS433_RS00305 (MAR054) 56059..56400 - 342 WP_012477176 P-type conjugative transfer protein TrbJ -
HS433_RS00310 (MAR055) 56330..56875 - 546 WP_000059835 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
HS433_RS00315 (MAR056) 56862..57146 - 285 WP_000624108 type-F conjugative transfer system pilin chaperone TraQ -
HS433_RS00320 (MAR057) 57227..57541 + 315 WP_000415570 hypothetical protein -
HS433_RS00325 (MAR058) 57543..57890 - 348 WP_001287909 conjugal transfer protein TrbA -
HS433_RS00330 (MAR059) 57906..58649 - 744 WP_001030379 type-F conjugative transfer system pilin assembly protein TraF traF
HS433_RS00335 (MAR060) 58642..58899 - 258 WP_000864318 conjugal transfer protein TrbE -
HS433_RS00340 (MAR061) 58927..60735 - 1809 WP_000821827 type-F conjugative transfer system mating-pair stabilization protein TraN traN
HS433_RS00345 (MAR062) 60732..61370 - 639 WP_000777697 type-F conjugative transfer system pilin assembly protein TrbC trbC
HS433_RS00350 (MAR063) 61379..62371 - 993 WP_000830821 conjugal transfer pilus assembly protein TraU traU
HS433_RS00355 (MAR064) 62368..63000 - 633 WP_001203740 type-F conjugative transfer system protein TraW traW
HS433_RS00360 (MAR065) 62997..63383 - 387 WP_000214079 type-F conjugative transfer system protein TrbI -
HS433_RS00365 (MAR066) 63380..66007 - 2628 WP_001064253 type IV secretion system protein TraC virb4
HS433_RS00370 (MAR067) 66167..66388 - 222 WP_001278689 conjugal transfer protein TraR -
HS433_RS00375 (MAR068) 66523..67038 - 516 WP_000809838 type IV conjugative transfer system lipoprotein TraV traV
HS433_RS00380 (MAR069) 67035..67286 - 252 WP_001038342 conjugal transfer protein TrbG -
HS433_RS00385 (MAR070) 67279..67599 - 321 WP_001057294 conjugal transfer protein TrbD virb4
HS433_RS00390 (MAR071) 67586..68176 - 591 WP_000002788 conjugal transfer pilus-stabilizing protein TraP -
HS433_RS00395 (MAR072) 68166..69593 - 1428 WP_000146685 F-type conjugal transfer pilus assembly protein TraB traB
HS433_RS00400 (MAR073) 69593..70321 - 729 WP_001230787 type-F conjugative transfer system secretin TraK traK
HS433_RS00405 (MAR074) 70308..70874 - 567 WP_000399792 type IV conjugative transfer system protein TraE traE
HS433_RS00410 (MAR075) 70896..71207 - 312 WP_000012106 type IV conjugative transfer system protein TraL traL
HS433_RS00415 (MAR076) 71222..71587 - 366 WP_000340270 type IV conjugative transfer system pilin TraA -
HS433_RS00420 (MAR077) 71621..71848 - 228 WP_012477178 conjugal transfer relaxosome protein TraY -
HS433_RS00425 (MAR078) 71943..72629 - 687 WP_000332491 PAS domain-containing protein -
HS433_RS00430 (MAR079) 72819..73202 - 384 WP_000124981 conjugal transfer relaxosome DNA-binding protein TraM -
HS433_RS00435 (MAR080) 73535..74137 + 603 WP_012477179 transglycosylase SLT domain-containing protein virB1
HS433_RS00440 (MAR081) 74433..75254 - 822 WP_001234454 DUF932 domain-containing protein -
HS433_RS00445 (MAR154) 75373..75659 - 287 Protein_89 hypothetical protein -
HS433_RS00450 75684..75803 - 120 WP_014106970 hypothetical protein -
HS433_RS00635 75855..76271 + 417 Protein_91 hypothetical protein -
HS433_RS00460 76360..76512 - 153 Protein_92 class I SAM-dependent methyltransferase -
HS433_RS00465 (MAR082) 76525..77898 - 1374 WP_000244358 IS4-like element ISEc13 family transposase -
HS433_RS00470 (MAR083) 78005..78481 - 477 WP_000556070 class I SAM-dependent methyltransferase -

Host bacterium

ID   757 GenBank   NC_010862
Plasmid name   pMAR7 Incompatibility group   IncFIB
Plasmid size   101558 bp Coordinate of oriT [Strand]   73269..73741 [+]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   blaTEM-2
Virulence gene   bfpA, bfpG, bfpB, bfpC, bfpU, bfpD, bfpE, bfpF, bfpP, bfpH, bfpI, bfpJ, bfpK, bfpL
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -