Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100288
• Name: oriT_pSMS35_8
• Organism: Escherichia coli SMS-3-5
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_010485 (2870..2955 [+], 86 nt)
• oriT length: 86 nt
• IRs (inverted repeats): 6..15, 19..28 (GTGTCGGGGC..GCCCTGACCC)
• Location of nic site: 59..60
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 86 nt

>oriT_pSMS35_8
AGGGGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAGTCATGCGGCATCGGTGCGGATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Nuclease_pSMS35_8

• ID: 359
• GenBank: WP_000635798
• Name: Nuclease_pSMS35_8
• UniProt ID: B1LRY7
• Length: 517 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 517 a.a.
• Molecular weight: 58151.55 Da
• Isoelectric Point: 5.7849

>WP_000635798.1 nuclease [Escherichia coli]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRTLTLPDNLPETKQAIAESVTRGLDVLYRAGEIKDRQDVIQALTDVGVEVVRVTRS
SISIADPNGGKNIRLKGAFYEQSFTDGRGVREKAERESRIYRESAEQRVQEARRICKRGCDIKRDENQRR
YSPVHSLDRGIAGETPGRGERGDEAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRY
QEAGRDIAETEREDMGRGVRAGREQEISGPSVREISRGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQEIRDELLIQE
REHELFLERERHLEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (57-237)

Protein structure

• AlphaFold DB: B1LRY7

Host bacterium

• ID: 750
• GenBank: NC_010485
• Plasmid name: pSMS35_8
• Incompatibility group: ColRNAI
• Plasmid size: 8909 bp
• Coordinate of oriT [Strand]: 2870..2955 [+]
• Host baterium: Escherichia coli SMS-3-5

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -