Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100282
• Name: oriT_pETEC_5
• Organism: Escherichia coli O139:H28 str. E24377A
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): NC_009791 (165..222 [+], 58 nt)
• oriT length: 58 nt
• IRs (inverted repeats): 1..10, 14..23 (GTGTCGGGGC..GCCATGACCC)
• Location of nic site: 54..55
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 58 nt

>oriT_pETEC_5
GTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGAAGTGTATACTGGCTTA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Nuclease_pETEC_5

• ID: 353
• GenBank: WP_000635799
• Name: Nuclease_pETEC_5
• UniProt ID: _
• Length: 517 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 517 a.a.
• Molecular weight: 57641.04 Da
• Isoelectric Point: 6.4550

>WP_000635799.1 nuclease [Escherichia coli]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRTLTLPDNLPETKQALAESVTRGIDALYHVGEIKGRQDVIQALTEAGLEVVRMTRS
SISIADPNGGKNIRLKGAFYEQSFTDGRGVREKAERESRIYRDNAERRVQQARKICKQGCDLKRDENQRR
YSPVHNVDRGIAGKTPGRGERGDDAAQEGRVKAGREYGHDVTGDGPFPVYREWRDALVSWRADTGEPGRN
QDTGRNIAETEREDMGRGVCAGREKEISGHPAGEIGNGDSLSGEGLGTTDGVTQSDGAGKTFAERLRATA
TGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQEIRDERLIQE
REHELSLERERQLEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (57-237)

Protein structure

No available structure.

Host bacterium

• ID: 744
• GenBank: NC_009791
• Plasmid name: pETEC_5
• Incompatibility group: ColRNAI
• Plasmid size: 5033 bp
• Coordinate of oriT [Strand]: 165..222 [+]
• Host baterium: Escherichia coli O139:H28 str. E24377A

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -