Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100279
Name   oriT_pLW1071 in_silico
Organism   Geobacillus thermodenitrificans NG80-2
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   NC_009329 (28359..28392 [+], 34 nt)
oriT length   34 nt
IRs (inverted repeats)      4..18, 22..34  (CCCATTTATACCCCT..AGGGGTAAATGGG)
Location of nic site      2..3nt
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 34 nt

>oriT_pLW1071
GTGCCCATTTATACCCCTTTCAGGGGTAAATGGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   350 GenBank   WP_011888495
Name   Nickase_pLW1071 insolico UniProt ID   A4IU06
Length   643 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 643 a.a.        Molecular weight: 75950.37 Da        Isoelectric Point: 9.5514

>WP_011888495.1 MobQ family relaxase [Geobacillus thermodenitrificans]
MAYFLFRTQIISKSNRSAVACAAYRSGEALYSERDGLTKKYGHRDVPPETYILAPKHAPDWVYNRERLWN
EVEKVEKQHNAQLAREIIVALPTQLTNEEQTQLLLDFCKENFADEGMVADIAIHRDKKHNPHAHIMLTMR
PFNEDGTWGKKRKKVNGKSVHLTNWNDKETLLKWRKNFADRINEKFKEKGIDDRVSHESYEKQGIDKVPQ
IRLSREAYQYEQRMKREAEKQGKPYEPVTYYGKLNQEIQCINQELKALKQKEKVVSISDYQEEKSLNETI
ESIRKNASLNDVEKASLLLVAKRAKTYVDFAVARRVYHDLAEGNWKKKIDSQQLKLRALKNVINKAYHVY
QQEPKQVIQYGFHPGKFNEQLREKIAQLKEMEKSFENELTKYEAVLKKAELALEVQKRLTEEEFRVLYPH
AGADFRIEEKYHAVQYFKDTGGVLPESEIPAYAAKKEQEIHRMPTFAEQTRNMYQSLFVLNRTIQKQSRE
RMEALKARDFEQAFEASRKIEQCMLRKEKMSKELEENKAVLQAILSEHYGRYVSITNTAALLQLHELVER
GESSKDLEADLRRIHVSEQQKLERMPKPETEAQIMEYQYRQHVADGLLEAIEEAQRANDRKKYEKDPTKK
RQRRRYRGQEFER

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(16-223)


  Protein structure


Source ID Structure
AlphaFold DB A4IU06


Host bacterium

ID   741 GenBank   NC_009329
Plasmid name   pLW1071 Incompatibility group   _
Plasmid size   57693 bp Coordinate of oriT [Strand]   28359..28392 [+]
Host baterium   Geobacillus thermodenitrificans NG80-2

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   involvement in alkane degradation
Symbiosis gene   -
Anti-CRISPR   -