Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100275
Name   oriT_pAOVO02 in_silico
Organism   Acidovorax sp. JS42
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_008766 (55155..55735 [-], 581 nt)
oriT length   581 nt
IRs (inverted repeats)      139..151, 157..169  (AGGCGCTGCCGG..CCGGCAGCGCCT)
 240..245, 250..255  (GCCGGC..GCCGGC)
 308..326, 329..347  (GCGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCGC)
Location of nic site      355..356
Conserved sequence flanking the
  nic site    		  TATCCTG|C
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 581 nt

>oriT_pAOVO02
CCCTTGAGCGCGTGCGCCGTGTGCCGCGCGTAGGTCGCCACGGCTCGCCAGTAGGCGGCCATCGGCCCCTTGCGGCGTGCCCATGCCTTCTCGGCTTCGTCGTTGGCCTGCGTGCGCAGCTCGCGCAGCACGCGGGCGAGGCGCTGCCGGTCGGCCACCGGCAGCGCCTGGAGTTCGCGGCCTGCTGGAAGCTCCAGCAGCGGATTGATGTAGCCCATGTCGTTCGTCTCTCGTTTCTTGCCGGCGGTAGCCGGCCGCCTCGCAGAGCAGGATGCCCGTTGAGTGCCGCAGGCGCGAATAAGGGACAGCGAAGATAGATAACCGGCTCGCCGGTTAGCTAACTTCGCCTATCCTGCCCGCCTTACGGCGTTGATAACACCAAGGAAAGTCTATACCGAAACCTTCGCATCTGTCTGCATTCAGCGCGAAAAGATGGCGGATATATCGAATGAAGGCGTACAATGCCTGGAAAGCGCGCTAGATGCAGCGGTGTCAGGCTGCTATCCACGCGCTTTACATAGACTTTCCACGGGTCGATACATGAAAATGACGGCTATTTACAAACCACGGAAGCGAGGCGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   346 GenBank   WP_011798731
Name   TraI_pAOVO02 insolico UniProt ID   A1WDT1
Length   752 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 752 a.a.        Molecular weight: 83050.85 Da        Isoelectric Point: 10.8263

>WP_011798731.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Burkholderiales]
MIVKHVPMRSLGKSDFAGLVNYVTDAQSKDHRLGQVQITNCDAMSVQDAITEVLATQHTNTRAKGDKTYH
LIVSFRAGEQPSADTLRAIEERICAGLGFAEHQRVSAVHNDTDNLHIHIAINKIHPTRHTMHEPFGQHRT
LAELCTALERDYGLERDNHEPRQRGAEGRAADMERHAGVESLVGWIKRECLDEIKGAQSWQELHQVMRDN
GLELRERANGLVIEAGDGTMVKASTLARDLSKPKLEARLGPFEPSPERQTRTKAKRQYQKDPIRLRVNTV
ELYARYKAEQQTLTATRGQALEQARRRKDRLVEAAKRSNRLRRATIKVVGESRTNKKLLYAQASKALRDE
IQAINKQYQQDRQALYDSHSRRTWADWLKKEATHGNAEALAALRAREAAQGLQGNTIQGRGDARPGHAPV
TDNITKKGTIIFRAGLSAVRDDGDKLQVSREATREGLQEALRLAMERYGSRITVNGTTQFKAQVIRAAVD
SQLPITFADPALESRRLALLKKENTHERPDRTERADQRPAEHRGRAGRGPGGPGQRAAAHDDAARPVAIA
RAGFGRPGAGRTDAAGVHAGAVHRKPDIGRIGRVPPPQSQHRLRTLSQLGVVRIAGGSEVLLPRDVPRHL
EQQGTQPDHQLRRGISRPGTGVATAPPGLAAADKYIAEREAKRSKGFDIPKHARYTDGDGALAFQGVRNI
EGQALALLKRGDEVMVMPIDQATARRLSRIAVGDAVTITPKGSIKTSKGRSR

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(420-507)

Relaxase/Mobilisation nuclease domain (Relaxase)

(12-251)


  Protein structure


Source ID Structure
AlphaFold DB A1WDT1


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 13139..23824

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AJS_RS22665 8168..8341 + 174 WP_172734609 hypothetical protein -
AJS_RS21170 (Ajs_4254) 8338..8544 + 207 WP_011798692 hypothetical protein -
AJS_RS21175 (Ajs_4255) 8608..8841 - 234 WP_011798693 hypothetical protein -
AJS_RS22230 (Ajs_4256) 8877..9074 + 198 WP_197953238 helix-turn-helix domain-containing protein -
AJS_RS22235 (Ajs_4257) 9010..9540 - 531 WP_011798694 hypothetical protein -
AJS_RS21185 (Ajs_4258) 9619..10293 - 675 WP_011798695 class I SAM-dependent methyltransferase -
AJS_RS21190 (Ajs_4259) 10449..10880 - 432 WP_001665065 OmpA family protein -
AJS_RS21195 (Ajs_4260) 10896..11594 - 699 WP_011798696 TraX family protein -
AJS_RS21200 (Ajs_4261) 11594..11860 - 267 WP_011798697 hypothetical protein -
AJS_RS21205 (Ajs_4262) 11889..12524 - 636 WP_011798698 transglycosylase SLT domain-containing protein -
AJS_RS21210 (Ajs_4263) 12538..13122 - 585 WP_011798699 TrbM/KikA/MpfK family conjugal transfer protein -
AJS_RS21215 (Ajs_4264) 13139..14872 - 1734 WP_011798700 P-type conjugative transfer protein TrbL virB6
AJS_RS21220 (Ajs_4265) 14883..15110 - 228 WP_011798701 entry exclusion lipoprotein TrbK -
AJS_RS21225 (Ajs_4266) 15120..15884 - 765 WP_011798702 P-type conjugative transfer protein TrbJ virB5
AJS_RS21230 (Ajs_4267) 15902..17320 - 1419 WP_011798703 TrbI/VirB10 family protein virB10
AJS_RS21235 (Ajs_4268) 17325..17813 - 489 WP_011798704 conjugal transfer protein TrbH -
AJS_RS21240 (Ajs_4269) 17816..18715 - 900 WP_000753228 P-type conjugative transfer protein TrbG virB9
AJS_RS21245 (Ajs_4270) 18733..19515 - 783 WP_011798705 conjugal transfer protein TrbF virB8
AJS_RS21250 (Ajs_4271) 19512..22070 - 2559 WP_011798706 VirB4 family type IV secretion/conjugal transfer ATPase virb4
AJS_RS21255 (Ajs_4272) 22067..22378 - 312 WP_001207961 conjugal transfer protein TrbD virB3
AJS_RS21260 (Ajs_4273) 22382..22846 - 465 WP_011222079 conjugal transfer system pilin TrbC virB2
AJS_RS21265 (Ajs_4274) 22859..23824 - 966 WP_011798707 P-type conjugative transfer ATPase TrbB virB11
AJS_RS21270 (Ajs_4275) 24135..24497 - 363 WP_000283041 transcriptional regulator -
AJS_RS21275 (Ajs_4276) 24606..24944 + 339 WP_011798708 single-stranded DNA-binding protein -
AJS_RS21280 (Ajs_4277) 24992..26215 + 1224 WP_011798709 plasmid replication initiator TrfA -
AJS_RS21285 (Ajs_4279) 26769..28640 + 1872 Protein_31 Tn3-like element IS1071 family transposase -

Host bacterium

ID   737 GenBank   NC_008766
Plasmid name   pAOVO02 Incompatibility group   IncP(Beta)
Plasmid size   63609 bp Coordinate of oriT [Strand]   55155..55735 [-]
Host baterium   Acidovorax sp. JS42

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -