Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100274
• Name: oriT_pNOCA01
• Organism: Nocardioides sp. JS614
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_008697 (124416..124925 [+], 510 nt)
• oriT length: 510 nt
• IRs (inverted repeats): IR1: 179..183, 185..189 (GGGCG..CGCCC) IR2: 225..237, 245..257 (GTTGGATTCGGCG..CGCCGAATCCACC)
• Location of nic site: 155..156
• Conserved sequence flanking the nic site: TACCCGCG|CCCT
• Note: predicted by the oriTfinder

oriT sequence

• Length: 510 nt

>oriT_pNOCA01
TTGAGGCCGCGGCCGCGAAGTCGCCGGCGGAACGCTGGGCCCCGCTGGCCCGCGAGGTCGACCCGCGCCTGGCGGAGCAGGACGACTGGCCCGCCACCGCCGCGATGCTGCAACAGGCCCACGAGCAAGGCCACGACGTCAGCGCCGCTACCCGCGCCCTGGTCGCAGCAGCACCCCTGGGCGACGCCCCCGCCCGCGAACTGCGCTACCGGCTGGTTCGGCAGGTTGGATTCGGCGAACACGACGCCGAATCCACCGACCGTGACGCCCCGAAGCGCCGGCGCGACGCGGGTACTGGACATGAGCTCGGCTCTTCGAACCCAGTGCACCGCAGCCGCCGCCGGTAGGCCTGGGCACAGGTTGAGACCTACAACGAGAGGGCTGTCGGACACTCAACGCGAGGTACCCGCGTGCAGACCTGCCACATTGCGGCCCACTGTGGGGCAGCGTCATGGCGCTCAAACGGGCGGGCTCCTTGTCCAGAGGTCTTCAAAGCGCCGGACTGGGCCG

oriT secondary structure

Predicted by RNAfold.

Relaxase

Tra_pNOCA01

• ID: 345
• GenBank: WP_011751509
• Name: Tra_pNOCA01
• UniProt ID: A1SC49
• Length: 1967 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 1967 a.a.
• Molecular weight: 212433.30 Da
• Isoelectric Point: 5.2135

>WP_011751509.1 MULTISPECIES: MobF family relaxase [unclassified Nocardioides]
MSLHKLTAGSGYDYLTRQVAAMDATDKGHTGLASYYTEKGETPGVWVGSGMEGIEGLDAGDVVTADHMQN
LFGAGHHPLATARTKELDLRVGRAGVGSPTAADYKAAARLGTPFKVYENDVSAFRVEVAKRLAAVNEAAG
LPGDWPVPAEERARIRTEIGRDFFRAEHGREPADARELAATIAKHSRPKTNAVAGYDLTFSPVKSVSTLW
ALADPKTAAVIEGAHQSAIKDALNFIETKALFTRQGTNGIRQVNVRGLLATAFTHRDSRAGDPDLHTHVA
VANKVQTLDGKWLAIDGRLLYKAKVSASETYNTALERHLGDALGVRFAERPNPDARKRPVREIVGVDPDL
NHRFSKRRVSVEARRKELAAQFQATHGRPPTPIETLQLAQRATLETREAKHEPRSLNDQREAWDREAVEV
LGSPRGLQQMLHGTLNPTAAGTAPVADAAWFAKTGDRIVTTMEGGRATWQYWHVYAEAQRQVRAANVPTD
QVSTVVGLLVSEVLDGRSVSITRPFDEISEPPELRKLDGASVYTVGGVHLFTSSKVLAAEERLVEAAGRR
DGYAVDEQNVALALLESEANGVTLNAGQATLVKEMATSGARLQLAIAPAGSGKTTAMRALAQAWTDGGGS
VIGLAPSAAAADALRVSMGSQIDTQTDTLAKLTHSLEQSQVTGAEVPDWVAGIGPQTLVVIDEAGMADTL
SLDIAVQHVLERGGSVRLVGDDQQLAAIGAGGVLRDIRATHGALQLTELVRFTDPAEGAASLALREGKGE
ALGFYLDRDRVHVGDLATMTEDVFAAWQADRAAGLDAIMLAPTRELVSELNQQARAHRLEGADPASVGPA
RRLADGNEASIGELIITRTNDRKLRTSATDWVKNGDRWTVLGLHEGGDLTVQHTQHGRTVRLPREYVETS
TELGYACTVHTAQGVTTDTMHGLATGNESRQQLYTMLTRGKSGNHIYLQVVGDGDPHSVIHPTLVRPLTP
TDILESMLARDDAQRSATSLMREQADPATQLGEAAQRYLDSLYVAAENHLRNTAGPDGSNVVEALDSTVD
QLLPGLADEAAWPTLRAHLLLLGAAGENPVEAMRAAASDRELVSAEDRAAVLDWRLDASGMRNVGTGPLP
WIPGVPERLADDPHWGAYLDQRAHLVDQLADEVRGRAAAQTSLPTWAQNGIRPDTDTVADVEVWRAAMQV
PLDDRRPTGAPQLQKASSTYQRRLNRRITGDHTPALTEWRQLLYSLAPQVRGDEFTPLLAERLAAMSRAG
VEAHQLLRTATAADTGAGPLPDEHAAAALWWRMARHLSPAVAAQVGDGSHGEGVTTSWAAQLPELLSDER
SARIQASAWWPALVSNIDHGLQRGWQLEALLSAGSALPSDDIDECQALVWRTSIALEPIPDEHEHDDHFD
EPPVDMWDGVEPDPATLVDHPDDTEWPLPHDLEADGDHLVDHPEPDEAIDQVDVAPAAADNVDEQQFVEG
DLTLAAYIRDLGGTPLEPTAADIRLMYQRADEWYSFPVTRERMIEINEITQTFFESRFTDSWGRDYLTGR
FGIDLAGHEHFRPGQAPAGWTNLVDHLRHRGVSGAEMLATGVATTASTGRLIDRFRDRVMFPVVHNGEVL
GFVGRRRPELTDADQANGRGGPKYLNTADTPLFHKGAQLYGVVDELLAEGAVPVIVEGPMDAIAVTLASA
ARYVGVAPLGTSLTDEQASQLGGIGRDPIVATDADLAGEVAAERDFWMLTPHGLDPGYARFPEGLDPADL
LAQRGPAALTASLASGQTLGDQLLSERLDNLAPEQARVAAMRVLAARPSRAWAPGTNLVRARLKLSQLQS
RRDLRDAIKAWDADPRRAARAELNSSSEVRARLEAAAAKSPAERWAPLAREVDPRLAEQDDWPATAAMLQ
QAHEQGHDVSAATRALVAAAPLGDAPARELRYRLVRQVGFGEHDAESTDRDAPKRRRDAGTGHELGSSNP
VHRSRRR

Protein domains

Predicted by InterproScan.

• DNA primase catalytic core, N-terminal domain (DNAG_N) (1526-1652)

• AAA domain (AAA_30) (583-785)

• TrwC relaxase (TrwC) (8-419)

• Toprim-like (Toprim_2) (1664-1751)

Protein structure

No available structure.

Host bacterium

• ID: 736
• GenBank: NC_008697
• Plasmid name: pNOCA01
• Incompatibility group: _
• Plasmid size: 307814 bp
• Coordinate of oriT [Strand]: 124416..124925 [+]
• Host baterium: Nocardioides sp. JS614

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: mercury resistance
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -