Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100271 |
| Name | oriT_pSK11-3  |
| Organism | Lactococcus cremoris subsp. cremoris SK11 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_008505 (29275..29737 [-], 463 nt) |
| oriT length | 463 nt |
| IRs (inverted repeats) | IR1: 138..147, 152..161 (CCCATTTTAT..ATAAAATGGG) IR2: 207..219, 223..235 (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: 242..255, 268..281 (ACATTGTAATACAA..TTGTATTACAATGT) |
| Location of nic site | 290..291 |
| Conserved sequence flanking the nic site |
CTTG|CA |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 463 nt
>oriT_pSK11-3
TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGGAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCAACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTATAGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATACTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGTAAGTTATTGATGGATCAGAAAAATGTATCACAAATTGAAACAAAG
TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGGAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCAACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTATAGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATACTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGTAAGTTATTGATGGATCAGAAAAATGTATCACAAATTGAAACAAAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 342 | GenBank | WP_011669060 |
| Name | Relaxase_pSK11-3 |
UniProt ID | Q02VG5 |
| Length | 135 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 135 a.a. Molecular weight: 15149.12 Da Isoelectric Point: 6.2189
>WP_011669060.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Lactococcus]
MTVIYMPRDTSGVIHSQGQLSRALNYIVNPEKTKGGELVSGQNINVPSNAYNEMLLTREMAILAGNQPKE
NERFGFHFVQSFSPEDNLTPEQVHEIGLKTMKAYLGESAEFVIATHTDKEHLHNVRPDRVLSQVV
MTVIYMPRDTSGVIHSQGQLSRALNYIVNPEKTKGGELVSGQNINVPSNAYNEMLLTREMAILAGNQPKE
NERFGFHFVQSFSPEDNLTPEQVHEIGLKTMKAYLGESAEFVIATHTDKEHLHNVRPDRVLSQVV
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q02VG5 |
Host bacterium
| ID | 733 | GenBank | NC_008505 |
| Plasmid name | pSK11-3 | Incompatibility group | _ |
| Plasmid size | 74750 bp | Coordinate of oriT [Strand] | 29275..29737 [-] |
| Host baterium | Lactococcus cremoris subsp. cremoris SK11 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |