Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100270
• Name: oriT_pMG828-3
• Organism: Escherichia coli
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_008488 (617..698 [-], 82 nt)
• oriT length: 82 nt
• IRs (inverted repeats): 3..12, 16..25 (GTGTCGGGGC..GCCCTGACCC)
• Location of nic site: 56..57
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 82 nt

>oriT_pMG828-3
GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGCCATCAGTGCGGAT

oriT secondary structure

Predicted by RNAfold.

Relaxase

pMG8283_03_pMG828-3

• ID: 341
• GenBank: YP_794134
• Name: pMG8283_03_pMG828-3
• UniProt ID: A0A1L2
• Length: 521 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 521 a.a.
• Molecular weight: 58735.10 Da
• Isoelectric Point: 6.2730

>YP_794134.1 hypothetical protein pMG8283_03 (plasmid) [Escherichia coli]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFANKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRILTLPDNLPETKQALAESVTRGIDALYHVGEIKGRQDVIQALTEAGLEVVRVTRS
SLSIADPNGGKNIRLKGAFYEQSFTDGRGVREKAERESRIYRDNAERRVQEARKICKQGCDIKRDENQRR
YSPVHSFDRGITEKTPGRGERGDDAAQEGRVKAGREYGHDVTGDGHFPVYREWRDALVSWRDDTGEPGRN
QEAERESGEAEREDMGRALSRGEQRTFSGVARGDESRNELDDGKRETECRKAGTGVTEHDGAGKTFAERI
RATATGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQEIRDER
LIQEREHALSLERERQLEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (51-237)

Protein structure

• AlphaFold DB: A0A1L2

Host bacterium

• ID: 732
• GenBank: NC_008488
• Plasmid name: pMG828-3
• Incompatibility group: ColRNAI
• Plasmid size: 5219 bp
• Coordinate of oriT [Strand]: 617..698 [-]
• Host baterium: Escherichia coli

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -