Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100269
Name   oriT_pO86A1 in_silico
Organism   Escherichia coli
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_008460 (64739..65280 [-], 542 nt)
oriT length   542 nt
IRs (inverted repeats)      243..255, 257..269  (TATTTAATAATTC..GAATTATTAAATA)
 311..318, 321..328  (GCAAAAAC..GTTTTTGC)
Location of nic site      337..338
Conserved sequence flanking the
  nic site    		  GTAGTGTGT|GG
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 542 nt

>oriT_pO86A1
TTACTCTGGCCATAAGATAAAGCCTTTCATTATTAAGCAATGAACTTTTCACTATAAATATGCATATAGTGTTTACAAGTAAGAAAGACACTCCTAGCAGCGCCTCTAGGATCATCCTATAAAAAAATGCGATCCGGCGCTAGGGGCGTCCCTAATATATATCAATGTTTTTCATGAAAATTGTCAGTACTGATCCTAATAAGAGTCGCTATAGGGTCGTAACAGGATCGCCAACGACTCTCTATTTAATAATTCAGAATTATTAAATATAAATAGCGTTTGTTAATTACATGATTTAAAACGCAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCCATCTGCCTGATGTTTATAAATGAGATCTGCCAGGCCACTGATTGCTTTGATCTTGCAGGCCGGGATTACAAAATAGATCCGGATTTACTGAGA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   340 GenBank   YP_788091
Name   TraI_pO86A1 insolico UniProt ID   Q08JE3
Length   1756 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191659.20 Da        Isoelectric Point: 5.7344

>YP_788091.1 conjugal transfer nickase/helicase TraI (plasmid) [Escherichia coli]
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSSRSQAIREAVGEGASLKSRDVAALDTRKSKQHVDPEIRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARVGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLQDGMTFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQILITDSGQRTGTGSALMSMKEAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQSIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRNAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFNMVDLLTEAKSFAAEGTGCTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVVIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGETFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISKDDGLITLEDAEGNTRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDCNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREVARENLLQERLQQMERDMVHDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(968-1156)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)

TrwC relaxase (TrwC)

(10-283)

DNA helicase TraI (TraI_C)

(1434-1557)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 64343..91291

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXB98_RS00340 (pO86A1_p083) 59926..60360 + 435 WP_011666470 conjugation system SOS inhibitor PsiB -
HXB98_RS00345 (pO86A1_p084) 60357..61119 + 763 Protein_75 plasmid SOS inhibition protein A -
HXB98_RS00755 61332..61445 + 114 Protein_76 DUF5431 family protein -
HXB98_RS00350 (pO86A1_p086) 61390..61512 + 123 WP_223200913 Hok/Gef family protein -
HXB98_RS00355 (pO86A1_p087) 61750..62062 - 313 Protein_78 hypothetical protein -
HXB98_RS00360 (pO86A1_p089) 62362..62664 + 303 WP_001272235 hypothetical protein -
HXB98_RS00365 (pO86A1_p090) 62820..63107 + 288 WP_011666474 hypothetical protein -
HXB98_RS00370 (pO86A1_p091) 63226..64047 + 822 WP_011666475 DUF932 domain-containing protein -
HXB98_RS00375 (pO86A1_p092) 64343..64945 - 603 WP_115189140 transglycosylase SLT domain-containing protein virB1
HXB98_RS00380 (pO86A1_p093) 65268..65651 + 384 WP_001354030 conjugal transfer relaxosome DNA-binding protein TraM -
HXB98_RS00385 (pO86A1_p094) 65845..66516 + 672 WP_011666477 conjugal transfer transcriptional regulator TraJ -
HXB98_RS00390 (pO86A1_p095) 66653..66880 + 228 WP_000089263 conjugal transfer relaxosome protein TraY -
HXB98_RS00395 (pO86A1_p096) 66913..67272 + 360 WP_011666478 type IV conjugative transfer system pilin TraA -
HXB98_RS00400 (pO86A1_p097) 67287..67598 + 312 WP_000012106 type IV conjugative transfer system protein TraL traL
HXB98_RS00405 (pO86A1_p098) 67620..68186 + 567 WP_000399805 type IV conjugative transfer system protein TraE traE
HXB98_RS00410 (pO86A1_p099) 68173..68901 + 729 WP_011666479 type-F conjugative transfer system secretin TraK traK
HXB98_RS00415 (pO86A1_p100) 68901..70352 + 1452 WP_011666480 F-type conjugal transfer pilus assembly protein TraB traB
HXB98_RS00420 (pO86A1_p101) 70342..70908 + 567 WP_011666481 conjugal transfer pilus-stabilizing protein TraP -
HXB98_RS00425 (pO86A1_p102) 70895..71212 + 318 WP_001057281 conjugal transfer protein TrbD -
HXB98_RS00430 (pO86A1_p103) 71212..71727 + 516 WP_011666482 type IV conjugative transfer system lipoprotein TraV traV
HXB98_RS00435 (pO86A1_p104) 71862..72083 + 222 WP_011666483 conjugal transfer protein TraR -
HXB98_RS00440 (pO86A1_p105) 72243..74870 + 2628 WP_011666484 type IV secretion system protein TraC virb4
HXB98_RS00445 (pO86A1_p106) 74867..75253 + 387 WP_000214084 type-F conjugative transfer system protein TrbI -
HXB98_RS00450 (pO86A1_p107) 75250..75882 + 633 WP_011666485 type-F conjugative transfer system protein TraW traW
HXB98_RS00455 (pO86A1_p108) 75879..76871 + 993 WP_000830839 conjugal transfer pilus assembly protein TraU traU
HXB98_RS00460 (pO86A1_p109) 76901..77206 + 306 WP_137519650 hypothetical protein -
HXB98_RS00465 (pO86A1_p110) 77215..77853 + 639 WP_011666487 type-F conjugative transfer system pilin assembly protein TrbC trbC
HXB98_RS00470 (pO86A1_p111) 77850..79700 + 1851 WP_011666488 type-F conjugative transfer system mating-pair stabilization protein TraN traN
HXB98_RS00475 (pO86A1_p112) 79727..79984 + 258 WP_032269641 conjugal transfer protein TrbE -
HXB98_RS00480 (pO86A1_p113) 79977..80720 + 744 WP_011666490 type-F conjugative transfer system pilin assembly protein TraF traF
HXB98_RS00485 (pO86A1_p114) 80736..81083 + 348 WP_011666491 conjugal transfer protein TrbA -
HXB98_RS00490 (pO86A1_p115) 81202..81486 + 285 WP_011666492 type-F conjugative transfer system pilin chaperone TraQ -
HXB98_RS00495 (pO86A1_p116) 81473..82018 + 546 WP_011666493 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
HXB98_RS00500 (pO86A1_p117) 81948..82295 + 348 WP_011666494 P-type conjugative transfer protein TrbJ -
HXB98_RS00505 (pO86A1_p118) 82276..82668 + 393 WP_242596732 F-type conjugal transfer protein TrbF -
HXB98_RS00510 (pO86A1_p119) 82655..84028 + 1374 WP_137519651 conjugal transfer pilus assembly protein TraH traH
HXB98_RS00515 (pO86A1_p120) 84025..86847 + 2823 WP_011666497 conjugal transfer mating-pair stabilization protein TraG traG
HXB98_RS00520 (pO86A1_p121) 86863..87348 + 486 WP_077513832 surface exclusion protein -
HXB98_RS00525 (pO86A1_p122) 87397..88128 + 732 WP_001403424 conjugal transfer complement resistance protein TraT -
HXB98_RS00530 (pO86A1_p123) 88332..88811 + 480 WP_001473214 hypothetical protein -
HXB98_RS00535 (pO86A1_p124) 89093..91291 + 2199 WP_011666500 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   731 GenBank   NC_008460
Plasmid name   pO86A1 Incompatibility group   IncFIB
Plasmid size   120730 bp Coordinate of oriT [Strand]   64739..65280 [-]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   aph(3')-IIa, aph(6)-Ic
Virulence gene   afaC-I, aap/aspU
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -