Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100256
Name   oriT_pCCK381 in_silico
Organism   Pasteurella multocida
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   NC_006994 (812..900 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)      5..10, 16..21  (GTTTCT..AGAAAC)
Location of nic site      32..33
Conserved sequence flanking the
  nic site    		  TAAGTGCG|CCCT
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 89 nt

>oriT_pCCK381
GGCAGTTTCTTGAAGAGAAACCGGTAAGTGCGCCCTCCCGTAAAATGACGGTCGGGGTTTGCGGTGTCTGTGCTCCGATGATAGCATAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   327 GenBank   YP_232869
Name   Mob_pCCK381 insolico UniProt ID   Q50I79
Length   703 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 703 a.a.        Molecular weight: 78829.72 Da        Isoelectric Point: 7.5023

>YP_232869.1 mobilization protein A (plasmid) [Pasteurella multocida]
MAIYHLTAKTGSRNGGQSAKAKADYIQREGRYSRDREEVLHTQSGHLPEWAERPADYWDGADLYERANGR
LFKEIEFALPVELTLDQQRELVDEFARHLTEGERLPYTLAIHAGNGENPHCHLMISERKNDGIERPADQW
FKRYNGKQPERGGAQKSESLKPKAWLEQTREAWSHYANRALEQAGHEARIDHRTLEAQGIERLPGIHLGP
NVVEMESRGIRTERADIALAIDTANGQIIDLQEYREVIEHERDRQSEEIQRDQRVSGRDRAAGPEHGDTG
GRSPAADRRGTAGQRGAGRELDEPATASHERMAAGSKGHEGSRRGTGQGRPDGPERRPWLDMEVVGRGLD
RFRDAYSGAADRIMALAGAANHRATGEHMADFQAQVKGDRTAQAIARQLKAMGCDRYDIGIRDAASGKMM
NREWTPQEVQQNAAWLKRMNAQGNDIYIRPAEQARHGLVLVDDLSSDDLDAMKQEGREPAAIIETSPKNY
QAWVKVAQDAPADHRGVIARKLAREYDADPASADSRHYGRLAGFTNRKDKYTSRTGYQPWVLCRESSGKS
ATAGPELMQQAGQVLDSIERRQERTARLAEITAPQSVRRYRRSVVDDYRSEMAGLVKRYGDDLSKCDFIA
AMKLASKGREPDEISKAMAEASPAIMERKAGHEADYIKRTVQKVMELPQVQEARAELAKQTQKQRSKGPD
LSM

  Protein domains


Predicted by InterproScan.

RepB DNA-primase from phage plasmid (RepB_primase)

(415-640)

MobA/MobL family (MobA_MobL)

(22-218)


  Protein structure


Source ID Structure
AlphaFold DB Q50I79


Host bacterium

ID   718 GenBank   NC_006994
Plasmid name   pCCK381 Incompatibility group   IncQ1
Plasmid size   10874 bp Coordinate of oriT [Strand]   812..900 [-]
Host baterium   Pasteurella multocida

Cargo genes

Drug resistance gene   floR
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -