Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100251
Name   oriT_pSERP in_silico
Organism   Staphylococcus epidermidis RP62A
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_006663 (22132..22369 [+], 238 nt)
oriT length   238 nt
IRs (inverted repeats)      IR1: 53..60, 62..69  (TTGGGGAT..ATCCCCAA)
  IR2: 95..99, 105..109  (TGGCT..AGCCA)
Location of nic site      116..117
Conserved sequence flanking the
  nic site    		  TGCTTG|CCA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 238 nt

>oriT_pSERP
AGTGGCTAGCATTTTGCCACTCATTTTTTGCTTTAGCAAAAACATAAGGGGCTTGGGGATTATCCCCAACAAGCCGGCGTCGCTACCACGTTAGTGGCTAGCAAAGCCAGTGCTTGCCAAACCACTTTATTACTTGATGAAATTTAGATTATCATATAATCTAAATTTATATTAAGTAATATCTTTCGTAGAAAGCGTGGTGGGATGTATGAGCGAACAAAAACATTTTGTGGCTAGC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   322 GenBank   WP_012817962
Name   Mob_pSERP insolico UniProt ID   _
Length   335 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 335 a.a.        Molecular weight: 38501.21 Da        Isoelectric Point: 6.2584

>WP_012817962.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Bacillales]
MATTKLSATKSTSRAINYAEKRAEEKSGLNCDIDYAKSSFKVTRELYGKTGGNEGHVIIQSFKPGEVTPE
QCNQLGLALAEKFAPNHQVAVYTHNDTDHVHNHIVINSIDLETGKKFNNNKQALRDLRDFNDNVCREHGL
SVPEKDTARLRYTQTEKAIADPNTKSTAQYSWKDEIREAIDQSKATNMDEFKDHLSQYGIEIARVTPKSI
TYRHLAEDKKVRGRRLGEDYNKGGIEDGFERQIQRQQQEREDDNDIQPKRPTSNRDEPTQRDTGVTQSDW
DKFAQDTNELERRRQAAESARLADEKARRDREERERAEQASRTIIDNDRDYGLEL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-242)


  Protein structure



No available structure.




Host bacterium

ID   713 GenBank   NC_006663
Plasmid name   pSERP Incompatibility group   _
Plasmid size   27310 bp Coordinate of oriT [Strand]   22132..22369 [+]
Host baterium   Staphylococcus epidermidis RP62A

Cargo genes

Drug resistance gene   blaZ, aph(3')-III, ant(6)-Ia
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21