Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100250
Name   oriT_pRK2 in_silico
Organism   Escherichia coli
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_005970 (1049..1223 [-], 175 nt)
oriT length   175 nt
IRs (inverted repeats)      2..11, 15..24  (GTTTCGGGGC..GCCCTGAACC)
Location of nic site      55..56
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 175 nt

>oriT_pRK2
GGTTTCGGGGCGAAGCCCTGAACCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTACTATGTTGGCATGGATGCGGAAATCAGTGAAGTGCTTCATGTGACAGGAGAAAAAAGGCGGCACAGGCGCGTCAGCAGAATATGCGATACAGGATATATTCCGCTTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   321 GenBank   YP_052693
Name   MobA_pRK2 insolico UniProt ID   Q6GUF9
Length   517 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 517 a.a.        Molecular weight: 57965.51 Da        Isoelectric Point: 6.1833

>YP_052693.1 putative mobilization protein MobA (plasmid) [Escherichia coli]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRTLILPDNLPETKQALAESVTRGLEVLYHAGEIKGRQDVIQALTEVGLEVVRVTRS
SISIADPNGGRNIRLKGAFYEQSFTDGRGVREKAERESRIYRENAERRIQQARRICQRGCDLKRDENQRR
YSPVYGLDGGIAEKTPGRGERGDDAAPEGHVKAGREFGHEVAGDSLSPVYREWRDALVSRREDTDEPGRN
QGTGRDIAETEREDMGCGVCSGRGKKISGHSARDVSRGDALSGEGLDTTDGVIRNDRVGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGTELERADRTLEPVIQRELEIREERLIQE
REHALSLERERQPEIQERTLDGPSLGW

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(57-237)


  Protein structure


Source ID Structure
AlphaFold DB Q6GUF9


Host bacterium

ID   712 GenBank   NC_005970
Plasmid name   pRK2 Incompatibility group   ColRNAI
Plasmid size   5360 bp Coordinate of oriT [Strand]   1049..1223 [-]
Host baterium   Escherichia coli

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -