Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100249
• Name: oriT_pSK639
• Organism: Staphylococcus epidermidis
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_005566 (173..410 [+], 238 nt)
• oriT length: 238 nt
• IRs (inverted repeats): IR1: 52..59, 61..68 (TTGGGGAT..ATCCCCAA) IR2: 94..98, 104..108 (TGGCT..AGCCA)
• Location of nic site: 115..116
• Conserved sequence flanking the nic site: TGCTTG|CCA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 238 nt

>oriT_pSK639
TGGCTAGCATTTAAATCACTCATTTTTTATGTAAGAAGAAACATAAGGGGCTTGGGGATTATCCCCAACAAGCAGGCGACACTACCACGTAAGTGGCTAGCAAAGCCAATGCTTGCCAAACCACTTAACGACTTGATGAATTTTACTTTATCCTATAAAGTAAAATGTAAGAAAGGCGTTATCTTTCGAAGAAAGCGTGGTGGGAATAATGAGCGAACAAAACACATTTGTGGCTAGC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Orf337_pSK639

• ID: 320
• GenBank: NP_976278
• Name: Orf337_pSK639
• UniProt ID: P95738
• Length: 337 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 337 a.a.
• Molecular weight: 38592.50 Da
• Isoelectric Point: 7.3199

>NP_976278.1 Orf337 (plasmid) [Staphylococcus epidermidis]
MMATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVIIQSFKPGEVTP
EQCNQLGLALAEKLVPNHQVAVYTHNDTDHVHNHIVINSIDLETGKKFNNNKQALRNVRNFNDEVCMEHG
LSVPEKNTARLRYTQTEKAIADPNTKSTAQYSWKDEIREAIDQSQATNMDEFKDHLNQHGIEIERVTPKS
ITYRHLAEDKKVRGRKLGEDYNKGGIEDGFERQIQHQQQTERVSDTDVQPKRRTSSRDESTERDTGVTQA
DWDQFAQDTNELERSRQAAESARLADEKARRDREKRERAKQTSRRIINNDRDHGLEL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (10-243)

Protein structure

• AlphaFold DB: P95738

Host bacterium

• ID: 711
• GenBank: NC_005566
• Plasmid name: pSK639
• Incompatibility group: _
• Plasmid size: 8013 bp
• Coordinate of oriT [Strand]: 173..410 [+]
• Host baterium: Staphylococcus epidermidis

Cargo genes

• Drug resistance gene: trimethoprim resistance
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -