Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100246
Name   oriT_pEL60 in_silico
Organism   Erwinia amylovora
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   NC_005246 (28663..28768 [+], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      18..35, 41..58  (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 106 nt

>oriT_pEL60
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   317 GenBank   NP_943241
Name   MobA_pEL60 insolico UniProt ID   Q6TFV4
Length   659 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75190.96 Da        Isoelectric Point: 10.0285

>NP_943241.1 mobility protein A (plasmid) [Erwinia amylovora]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVN
VEPGVQRVVVGDVTCQYNTFSLDGAALEMNSVSQQNTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB Q6TFV4


T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 31564..59193

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS249_RS00245 (pEL60p38) 26649..26960 + 312 WP_011154461 hypothetical protein -
HS249_RS00250 (pEL60p39) 27093..27629 + 537 WP_011091066 hypothetical protein -
HS249_RS00255 (pEL60p40) 28130..28525 - 396 WP_172682140 MobC -
HS249_RS00415 28524..29087 + 564 WP_020277892 plasmid mobilization protein MobA -
HS249_RS00265 (pEL60p42) 29074..31053 + 1980 WP_011154463 TraI/MobA(P) family conjugative relaxase -
HS249_RS00270 (pEL60p43) 31067..31567 + 501 WP_004187320 DotD/TraH family lipoprotein -
HS249_RS00275 (pEL60p44) 31564..32343 + 780 WP_011154464 type IV secretory system conjugative DNA transfer family protein traI
HS249_RS00280 (pEL60p45) 32354..33517 + 1164 WP_011154465 plasmid transfer ATPase TraJ virB11
HS249_RS00285 33507..33767 + 261 WP_168397230 IcmT/TraK family protein traK
HS249_RS00420 33792..37172 + 3381 WP_011154466 LPD7 domain-containing protein -
HS249_RS00295 (pEL60p47) 37096..37650 + 555 WP_011154467 hypothetical protein traL
HS249_RS00300 37651..37848 - 198 WP_004187464 Hha/YmoA family nucleoid-associated regulatory protein -
HS249_RS00305 37835..38245 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
HS249_RS00310 (pEL60p49) 38244..39026 + 783 WP_004206929 DotI/IcmL family type IV secretion protein traM
HS249_RS00315 (pEL60p50) 39035..40186 + 1152 WP_011154469 DotH/IcmK family type IV secretion protein traN
HS249_RS00320 (pEL60p51) 40198..41547 + 1350 WP_011154470 conjugal transfer protein TraO traO
HS249_RS00325 (pEL60p52) 41559..42263 + 705 WP_011154471 conjugal transfer protein TraP traP
HS249_RS00330 (pEL60p53) 42287..42817 + 531 WP_011091077 conjugal transfer protein TraQ traQ
HS249_RS00335 (pEL60p54) 42834..43223 + 390 WP_011154472 DUF6750 family protein traR
HS249_RS00340 (pEL60p55) 43269..43763 + 495 WP_011154473 hypothetical protein -
HS249_RS00345 (pEL60p56) 43760..46810 + 3051 WP_011154474 conjugative transfer protein traU
HS249_RS00350 (pEL60p57) 46807..48015 + 1209 WP_011091082 conjugal transfer protein TraW traW
HS249_RS00355 (pEL60p58) 48012..48662 + 651 WP_011154475 hypothetical protein -
HS249_RS00360 (pEL60p59) 48655..50835 + 2181 WP_004187492 DotA/TraY family protein traY
HS249_RS00365 (pEL60p60) 50838..51491 + 654 WP_011154477 hypothetical protein -
HS249_RS00370 (pEL60p61) 51553..51795 + 243 WP_023893611 IncL/M type plasmid replication protein RepC -
HS249_RS00375 (pEL60p63) 52081..53148 + 1068 WP_168397220 plasmid replication initiator RepA -
HS249_RS00380 54153..54290 + 138 Protein_75 type II toxin-antitoxin system CcdA family antitoxin -
HS249_RS00425 54679..54774 + 96 WP_004206884 DinQ-like type I toxin DqlB -
HS249_RS00385 (pEL60p64) 54825..56912 - 2088 WP_011154480 conjugative transfer protein -
HS249_RS00390 (pEL60p65) 56925..57875 - 951 WP_168397222 DsbC family protein trbB
HS249_RS00395 (pEL60p66) 57886..59193 - 1308 WP_011154482 hypothetical protein trbA
HS249_RS00400 (pEL60p67) 59193..59588 - 396 WP_011154483 lytic transglycosylase domain-containing protein -
HS249_RS00405 (pEL60p68) 59693..60088 - 396 WP_011154484 DUF1496 domain-containing protein -

Host bacterium

ID   708 GenBank   NC_005246
Plasmid name   pEL60 Incompatibility group   IncL/M
Plasmid size   60145 bp Coordinate of oriT [Strand]   28663..28768 [+]
Host baterium   Erwinia amylovora

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -