Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100244
• Name: oriT_pSCFS1
• Organism: Mammaliicoccus sciuri
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): NC_005076 (15358..15395 [-], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): 1..6, 10..15 (CTTTAT..ATAAAG)
• Location of nic site: 24..25
• Conserved sequence flanking the nic site: GTG|TTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 38 nt

>oriT_pSCFS1
CTTTATGGAATAAAGTATAGTGTGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

ORF5_pSCFS1

• ID: 315
• GenBank: NP_899168
• Name: ORF5_pSCFS1
• UniProt ID: Q7WVB7
• Length: 392 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 392 a.a.
• Molecular weight: 46246.65 Da
• Isoelectric Point: 9.0196

>NP_899168.1 putative recombinase/mobilization protein (plasmid) [Staphylococcus sciuri]
MQGFVAEPIASQSTHFMDIKYSLLYFTWKLTELWGCIFVSFAVVRMQKMKSPDLKGMQFHNQRERESRTN
PDIDPDRAHFNYDLLHQEKIDYNKQVKAIIESQKVSERKTRKDAVLVNELLVTSDRKFFDGLDPAEQKRF
FEESYKLFSERYGKQNIAYATVHNDEKTPHMHLGVVPMRDGKLQGKNIFNRQELQWMQEEFPKHMQSVGF
EVERGIASDRKHIEMSRFKALTLNEEIKTLEKETEVLRNALTASKKVDELQVSKPSLFDRNHVKLPVEDF
EALKARAKATEAIESTIEAHEKRFDDMVDNVIASDRKLDQEKTKTAQLQKENSGLKKENLELQKENKTLK
SQLNVLMEFAKSQLEKFKEWQKERQQEKEKNIARKRDQELER

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (41-230)

Protein structure

• AlphaFold DB: Q7WVB7

Host bacterium

• ID: 706
• GenBank: NC_005076
• Plasmid name: pSCFS1
• Incompatibility group: _
• Plasmid size: 17108 bp
• Coordinate of oriT [Strand]: 15358..15395 [-]
• Host baterium: Mammaliicoccus sciuri

Cargo genes

• Drug resistance gene: resistance to florfenicol,chloramphenicol, MLS_B(macrolidelincosamide-streptogramin B), spectinomycin
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -