Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100242
• Name: oriT_pLG13
• Organism: Escherichia coli
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_005019 (834..915 [-], 82 nt)
• oriT length: 82 nt
• IRs (inverted repeats): 3..10, 17..24 (GTGTCGGG..CCTGACCC)
• Location of nic site: 55..56
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 82 nt

>oriT_pLG13
GGGTGTCGGGGCGCAGCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGGCATCAGTGCGGATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

MobA_pLG13

• ID: 313
• GenBank: NP_863577
• Name: MobA_pLG13
• UniProt ID: Q9F899
• Length: 517 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 517 a.a.
• Molecular weight: 58215.71 Da
• Isoelectric Point: 6.2323

>NP_863577.1 hypothetical protein pLG13_03 (plasmid) [Escherichia coli]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFIIPNMELQTGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRILTLSDNLPETKQALAESVTRWIDALYHVGEIKGRQDVIQALTEAGIEVVRVTRS
SISIADPNGGRNIRLKGAFYEQSFTDGRGVREKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRR
YSPVHSLDREIAGKTQGRGERGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRN
QEAGRDIAQTEREDMGRGVCAGREQEISGPSVREISRGDSLSGERVGTSEGVTQNDRAGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQRELEIREERLIQE
REHALSLERERQLEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (57-237)

Protein structure

• AlphaFold DB: Q9F899

Host bacterium

• ID: 704
• GenBank: NC_005019
• Plasmid name: pLG13
• Incompatibility group: ColRNAI
• Plasmid size: 6293 bp
• Coordinate of oriT [Strand]: 834..915 [-]
• Host baterium: Escherichia coli

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -