Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100240
• Name: oriT_pSE-12228-02
• Organism: Staphylococcus epidermidis ATCC 12228
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): NC_005007 (3054..3106 [+], 53 nt)
• oriT length: 53 nt
• IRs (inverted repeats): 12..19, 35..42 (GTATATTC..GAATATAC)
• Location of nic site: 50..51
• Conserved sequence flanking the nic site: CGCTTG|CCA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 53 nt

>oriT_pSE-12228-02
CCATCAAGCCGGTATATTCAGAACGAAGTGGCTAGAATATACGACGCTTGCCA

oriT secondary structure

Predicted by RNAfold.

Relaxase

RLX_pSE-12228-02

• ID: 311
• GenBank: NP_863255
• Name: RLX_pSE-12228-02
• UniProt ID: A0A0H2VL15
• Length: 283 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 283 a.a.
• Molecular weight: 32832.45 Da
• Isoelectric Point: 6.9149

>NP_863255.1 RLX protein (plasmid) [Staphylococcus epidermidis ATCC 12228]
MATTKLGNTKSASRAINYAEKRAEEKSGLNCDIDYAKSSFKQTRALYGKENGVQAHTVIQSFKPGEVTPE
QCNQLGLELAEKIAPNHQVAVYTHADTNHVHNHIVINSIDLETGKKYQSNKKQREFVKQKNDEVCREHGL
SVTERNATMRYTQAEKGLIEKDKSSWKDEIRDAIEQSQATNFEELERELNQLDITIDRVTNKTITYRHLK
ENKKVRGKNLGEQFDKGALENEFAVANERREFEQQLSEYEPRESEYRPRDTEKNIKRPKELRVQSRENDF
ELE

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (8-233)

Protein structure

• AlphaFold DB: A0A0H2VL15

Host bacterium

• ID: 702
• GenBank: NC_005007
• Plasmid name: pSE-12228-02
• Incompatibility group: _
• Plasmid size: 4679 bp
• Coordinate of oriT [Strand]: 3054..3106 [+]
• Host baterium: Staphylococcus epidermidis ATCC 12228

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -