Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100239 |
| Name | oriT_pSE-12228-06  |
| Organism | Staphylococcus epidermidis ATCC 12228 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_005003 (1267..1506 [+], 240 nt) |
| oriT length | 240 nt |
| IRs (inverted repeats) | IR1: 1..8, 14..21 (AGTGGCTA..TAGCCACT) IR2: 22..29, 34..41 (ATTTTTTC..GAAAAAAT) IR3: 95..99, 105..109 (TGGCT..AGCCA) |
| Location of nic site | 116..117 |
| Conserved sequence flanking the nic site |
TGCTTG|CCA |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 240 nt
>oriT_pSE-12228-06
AGTGGCTAGCAATTAGCCACTATTTTTTCGTTAGAAAAAATCCATAAGGGGCTTGGGGATTACCCCCAACAAGCAGGCGCGTCTGCCACGTTAGTGGCTAGCAAAGCCAATGCTTGCCAAACCACTTAACGACTTGATGAATCTTACTTTATCATATAAAGTAAGATGTAAGAACGGCGTTATCTTTCGAAGAAAGCGTGGTGGGATTGAATGAGCGAACAAAACACATTTGTGGCTAGC
AGTGGCTAGCAATTAGCCACTATTTTTTCGTTAGAAAAAATCCATAAGGGGCTTGGGGATTACCCCCAACAAGCAGGCGCGTCTGCCACGTTAGTGGCTAGCAAAGCCAATGCTTGCCAAACCACTTAACGACTTGATGAATCTTACTTTATCATATAAAGTAAGATGTAAGAACGGCGTTATCTTTCGAAGAAAGCGTGGTGGGATTGAATGAGCGAACAAAACACATTTGTGGCTAGC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 310 | GenBank | NP_863197 |
| Name | Mob_pSE-12228-06 |
UniProt ID | A0A0H2VJA7 |
| Length | 336 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 336 a.a. Molecular weight: 38414.55 Da Isoelectric Point: 9.8063
>NP_863197.1 mobilization protein (plasmid) [Staphylococcus epidermidis ATCC 12228]
MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKAARELYGKTDGNQGHVIIQSFQPGEVTPE
QCNQLGLALAEKLVPNHQVAVYTHADTNHVHNHIVLNSIDLNTGKKFNNNKQALRDVRAFNDEVCRAQGL
SVPEKDTARLRYTQTEKAIADPNTTSTAQYSWKDDIREAIDQSTATNMEEFQKHLNQHGITIERVTPKSI
TYRHLAENKKVRGRKLGEDYNKGGIQHGFERQIQRRQQQKHKSDTNFQPKRSTSNRHESTERDTGVTQSD
WDQFAQDTNELERRRQAAESARLVDEKARRDREKRVREKPTSRPIINIDRDHGLEL
MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKAARELYGKTDGNQGHVIIQSFQPGEVTPE
QCNQLGLALAEKLVPNHQVAVYTHADTNHVHNHIVLNSIDLNTGKKFNNNKQALRDVRAFNDEVCRAQGL
SVPEKDTARLRYTQTEKAIADPNTTSTAQYSWKDDIREAIDQSTATNMEEFQKHLNQHGITIERVTPKSI
TYRHLAENKKVRGRKLGEDYNKGGIQHGFERQIQRRQQQKHKSDTNFQPKRSTSNRHESTERDTGVTQSD
WDQFAQDTNELERRRQAAESARLVDEKARRDREKRVREKPTSRPIINIDRDHGLEL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H2VJA7 |
Host bacterium
| ID | 701 | GenBank | NC_005003 |
| Plasmid name | pSE-12228-06 | Incompatibility group | _ |
| Plasmid size | 6585 bp | Coordinate of oriT [Strand] | 1267..1506 [+] |
| Host baterium | Staphylococcus epidermidis ATCC 12228 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |