Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100237 |
| Name | oriT_pAH82  |
| Organism | Lactococcus lactis subsp. lactis |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_004966 (4264..4722 [+], 459 nt) |
| oriT length | 459 nt |
| IRs (inverted repeats) | IR1: 138..147, 152..161 (CCCATTTTAT..ATAAAATGGG) IR2: 207..219, 223..235 (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: 242..255, 268..281 (ACATTGTAATACAA..TTGTATTACAATGT) |
| Location of nic site | 290..291 |
| Conserved sequence flanking the nic site |
CTTA|CA |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 459 nt
>oriT_pAH82
TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGGAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCAACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTACAGTATTTATGGTTTTATATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGGAAGTTCTTGATGGATCAGAAAAATGTATCACAAATTTAAAC
TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGGAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCAACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTACAGTATTTATGGTTTTATATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGGAAGTTCTTGATGGATCAGAAAAATGTATCACAAATTTAAAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 308 | GenBank | NP_862609 |
| Name | Mob_pAH82 |
UniProt ID | Q9FB66 |
| Length | 135 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 135 a.a. Molecular weight: 15462.52 Da Isoelectric Point: 6.4884
>NP_862609.1 relaxase Mob DEI (plasmid) [Lactococcus lactis subsp. lactis]
MTVIYMPKQSNGTVHSSKDLNQLIDYVMNPEKTNDFEYVSGQNILDIHSTCDEMLATRTMAIALKNKPRK
NEIYGYHFVQSFSPDDHLTPEQVHEIGCKTMKEYLGNSAEFIIATHTDKPHLHNVRPDRVLSQVV
MTVIYMPKQSNGTVHSSKDLNQLIDYVMNPEKTNDFEYVSGQNILDIHSTCDEMLATRTMAIALKNKPRK
NEIYGYHFVQSFSPDDHLTPEQVHEIGCKTMKEYLGNSAEFIIATHTDKPHLHNVRPDRVLSQVV
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q9FB66 |
Host bacterium
| ID | 699 | GenBank | NC_004966 |
| Plasmid name | pAH82 | Incompatibility group | _ |
| Plasmid size | 20331 bp | Coordinate of oriT [Strand] | 4264..4722 [+] |
| Host baterium | Lactococcus lactis subsp. lactis |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | cadmium resistance |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |