Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100236
• Name: oriT_pBLO1
• Organism: Bifidobacterium longum NCC2705
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): NC_004943 (3560..3591 [+], 32 nt)
• oriT length: 32 nt
• IRs (inverted repeats): 1..8, 12..19 (ATGCAACC..GGTTGCAT)
• Location of nic site: 28..29
• Conserved sequence flanking the nic site: TAAGTGCG|CCCT
• Note: predicted by the oriTfinder

oriT sequence

• Length: 32 nt

>oriT_pBLO1
ATGCAACCTCCGGTTGCATGTAAGTGCGCCCT

oriT secondary structure

Predicted by RNAfold.

Relaxase

MobA_pBLO1

• ID: 307
• GenBank: NP_862283
• Name: MobA_pBLO1
• UniProt ID: Q8GN32
• Length: 565 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 565 a.a.
• Molecular weight: 64293.64 Da
• Isoelectric Point: 9.5234

>NP_862283.1 mobilization protein MobA (plasmid) [Bifidobacterium longum NCC2705]
MAIYHLSVSNVSRASGSRATATLSYITGKRVHDERRGETYDYGRKERVLRVGTLLPEGAPAEFADPAVLF
NAVELHETGRTARPAKKIVVALPREFTPRQRVQALEGYIRENLNADGYAATYAIHDDGQGNNPHAHILVA
NRQIDPKTGGWAKLKQRMEYVLDERGERVPLIDPKTGAQKLDKRGRRQWKRTSVSLNPLDRKAKLQSLRE
SWAKTCNARLDETARIDHRSLEDQGSDLEPTIHEGYAARAIERAGGVSERCQTNREIRRSNDLLTAIRHE
LGRIFDRLGELFAAKIGQLRQRQARPEAAREPNWRYFEGDARRQLEADRADHAAALRGRLMDARADVRNR
ETWWKNHGTEEFEAAKQIIDVACEASREARDAGMFQRGRLRRNAEQVAGEQSERLRGAVPWLEDTEIPAD
WDQANRFRMRTTKAIHDHDMQPRTGLVAELERQLEQAEQAAGERPSPEQVKDLALRMAEQRERAEGQARG
ANRPEPSGDEPLDLSEPEDWGESSPFEQRATPKTKADLLGELKQRADRRTGRQEAEQRQIDPWSAQPPQR
RGHGR

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (19-253)

Protein structure

• AlphaFold DB: Q8GN32

Host bacterium

• ID: 698
• GenBank: NC_004943
• Plasmid name: pBLO1
• Incompatibility group: _
• Plasmid size: 3626 bp
• Coordinate of oriT [Strand]: 3560..3591 [+]
• Host baterium: Bifidobacterium longum NCC2705

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -