Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100235
• Name: oriT_PNAC2
• Organism: Bifidobacterium longum
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): NC_004769 (1082..1113 [-], 32 nt)
• oriT length: 32 nt
• IRs (inverted repeats): 1..8, 12..19 (ATGCAACC..GGTTGCAT)
• Location of nic site: 28..29
• Conserved sequence flanking the nic site: TAAGTGCG|CCCT
• Note: predicted by the oriTfinder

oriT sequence

• Length: 32 nt

>oriT_PNAC2
ATGCAACCTCCGGTTGCATGTAAGTGCGCCCT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Mob_PNAC2

• ID: 306
• GenBank: NP_848160
• Name: Mob_PNAC2
• UniProt ID: Q83YJ4
• Length: 565 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 565 a.a.
• Molecular weight: 64666.07 Da
• Isoelectric Point: 9.7356

>NP_848160.1 Mob (plasmid) [Bifidobacterium longum]
MAIYHLSVSNVSRASGSKATATLSYISGRRVHDERRGETYDYGRKERVLRVGTLLPEGAPAEFADPAVLF
NAVELHETGRTARPAKKIVVALPREFTPRQRVQALEEYIRENLNADGYAATYAIHEDREGNNPHAHILVA
NRQIDPATGGWARLKQRMEYVLDERGERVPLIDPETGRQKTDKRGRRQWKRTSVSLNPLDRKAKLKALRE
SWANTCNARLDETARIDHRSLEDQGSDLEPTIHEGYAARAIERAGGVSERCQTNREIRRSNSLLTAIRTE
LGRIFDRLGELFAAKIRQLRQRQARPEPAREPNWRYFEGDARRQLEADKADHTAAIRGKLMDARADVGNR
EEWWRSHGTEEHEAAKQIIDVARAASREARDANIFKRGRLLRNAEQVAGEQSEKLRGAVPWLEDTDIPAD
WDQANRFRMRTTKAIYDHDMQPRTRLVAELERRLEQAERAESERPSPEQVKALALRMAEQRAQAENPTQD
TKRPAPSGDEPLDLSEPEDWGEASPFESRQAPRTKADLLNEIRQRTDRLTGQREAEQRQIDPWSAQPPQR
RGHGR

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (19-253)

Protein structure

• AlphaFold DB: Q83YJ4

Host bacterium

• ID: 697
• GenBank: NC_004769
• Plasmid name: PNAC2
• Incompatibility group: _
• Plasmid size: 3684 bp
• Coordinate of oriT [Strand]: 1082..1113 [-]
• Host baterium: Bifidobacterium longum

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -