Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100233
• Name: oriT_pB44
• Organism: Bifidobacterium longum
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): NC_004443 (3480..3511 [+], 32 nt)
• oriT length: 32 nt
• IRs (inverted repeats): 1..8, 12..19 (ATGCAACC..GGTTGCAT)
• Location of nic site: 28..29
• Conserved sequence flanking the nic site: TAAGTGCG|CCCT
• Note: predicted by the oriTfinder

oriT sequence

• Length: 32 nt

>oriT_pB44
ATGCAACCTCCGGTTGCATGTAAGTGCGCCCT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Mob_pB44

• ID: 304
• GenBank: YP_001469635
• Name: Mob_pB44
• UniProt ID: A7X7J0
• Length: 566 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 566 a.a.
• Molecular weight: 64703.15 Da
• Isoelectric Point: 9.3423

>YP_001469635.1 putative mobilization protein (plasmid) [Bifidobacterium longum]
MAIYHLSVSNVSRASGSRATATLSYITGKRVHDERRGETYDYGRKERVLRVGTLLPEGAPAEFADPAVLF
NAVELHETGRTARPAKKIVVALPREFTPRQRVQALEEYIRENLNADGYAATYAIHEDREGNNPHAHILVA
NRQIDPATGGWARLKQRMEYVLDERGERVPLIDPETGRQKTDKRGRRQWKRTSVSLNPLDRKAKLKALRE
SWAKTCNARLDETARIDHRSLEDQGSDLEPTIHEGYAARAIERAGGVSERCEANREIRRSNGLLTAIRTE
LGRIFDRLGELFAAKIRQLRQRQARPEPAREPNWRYFEGDARRQLEADRADHTAAIRGKLMGAKADVRNR
EEWWRSHGTEEFEAAKQIIDVARAASREARDANVFKRGRLLRNAEQVAGEQSEKLRGAVPWLEDTDIPSD
WDQANRFRMRATKAIHDHDMQPRTGLVDELERRLEQAERAEGEKPSPEQVKALALRMAEQRERAAEDPTQ
DMNRPEPSGDEPLDLSEPEDLGEGGPFEERSAPRTKADLLREIRQRADRRTGQQEAEQRQIDPWQAQPPQ
RRGHGR

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (19-253)

Protein structure

• AlphaFold DB: A7X7J0

Host bacterium

• ID: 695
• GenBank: NC_004443
• Plasmid name: pB44
• Incompatibility group: _
• Plasmid size: 3624 bp
• Coordinate of oriT [Strand]: 3480..3511 [+]
• Host baterium: Bifidobacterium longum

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -