Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100231
• Name: oriT_pC
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_003457 (749..833 [-], 85 nt)
• oriT length: 85 nt
• IRs (inverted repeats): _
• Location of nic site: 59..60
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 85 nt

>oriT_pC
AGCGGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGGCATCAGTGCAGAT

oriT secondary structure

Predicted by RNAfold.

Relaxase

MbeA_pC

• ID: 302
• GenBank: NP_604403
• Name: MbeA_pC
• UniProt ID: Q8RLL1
• Length: 533 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 533 a.a.
• Molecular weight: 59519.17 Da
• Isoelectric Point: 6.0809

>NP_604403.1 MbeA (plasmid) [Salmonella enterica subsp. enterica serovar Enteritidis]
MSCAGCVWPSGNRGRGMIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYT
SGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYY
DRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQALAESVTRGIDALYHAGEIKGRQDVI
QALTEAGLEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFTDGRGVREKAERESRIYRENAERRVQEARR
VCQRGCDLKRDENQRRYSPVHSLDREIAGKTPGRGERGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWR
DALVSWRTDTDEPGRNQEAGRDIAETEREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQ
SDRAGNTFAERLRAAATGLYAAAERMGELLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEP
VIQRELEIREERLIQEREHALSLERERQPEIQERTLDGPSLGW

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (73-253)

Protein structure

• AlphaFold DB: Q8RLL1

Host bacterium

• ID: 693
• GenBank: NC_003457
• Plasmid name: pC
• Incompatibility group: ColRNAI
• Plasmid size: 5269 bp
• Coordinate of oriT [Strand]: 749..833 [-]
• Host baterium: Salmonella enterica subsp. enterica serovar Enteritidis

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -