Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100230
Name   oriT_pK in_silico
Organism   Salmonella enterica subsp. enterica serovar Enteritidis
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   NC_003456 (601..774 [-], 174 nt)
oriT length   174 nt
IRs (inverted repeats)      2..11, 15..24  (GTTTCGGGGC..GCCCTGAACC)
Location of nic site      55..56
Conserved sequence flanking the
  nic site    		  CTGG|CTTA
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 174 nt

>oriT_pK
GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGCACGGGTGCGGGAATCAGTGAAGCGCTTCATGTGGCAGGAGAAAAATGCAGCAGCGGAGCGTAAGCAGAATATATGAGACAGGATATATTCCGCTTCCTCGCTCAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   301 GenBank   NP_604399
Name   MbeA_pK insolico UniProt ID   Q8RLL4
Length   515 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 515 a.a.        Molecular weight: 56891.56 Da        Isoelectric Point: 8.4653

>NP_604399.1 MbeA (plasmid) [Salmonella enterica subsp. enterica serovar Enteritidis]
MPDSSGCGMPCWKRVQTMIVKFHPRGRGGGAGPVDYLLGKDRQRDGATVLQGKPEEVRELIDASPYAKKY
TSGVLSFAEKDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHQDKGRLELNFLIPNTELLTGRRLQPY
YDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSALPETKQEAAQAITRGLLALASSGELKTRQDV
TEALESAGFEVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGKGLRAEIESAAAEYRRDAESRIQRAR
EVCQSGTERKREENQRRHPRPRPEAVLSHEPAHERHGAYGQPDVADHRPGVCTADSIGRKHSVVAGAADT
RQLHDYPGAEAHAGHAVREEQRRAALDLRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDGAGKTVAER
IRAATAGLLEKAGRVGERLRGMADDVWSYATGERGAERARHGLEQAGAEFERSAAPVVERLNSIEAHRQQ
ERAAQHQKTLELERSQRQKGFELGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(74-255)


  Protein structure


Source ID Structure
AlphaFold DB Q8RLL4


Host bacterium

ID   692 GenBank   NC_003456
Plasmid name   pK Incompatibility group   Col440I
Plasmid size   4245 bp Coordinate of oriT [Strand]   601..774 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Enteritidis

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -