Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100229 |
| Name | oriT_pSLT  |
| Organism | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | NC_003277 (60667..60925 [-], 259 nt) |
| oriT length | 259 nt |
| IRs (inverted repeats) | 115..128, 134..147 (ATAGCGGTGCCGGC..GCCTGCACCGCTAT) |
| Location of nic site | 241..242 |
| Conserved sequence flanking the nic site |
GTGGGGTGT|GG |
| Note | predicted by the oriTfinder |
oriT sequence
Download Length: 259 nt
>oriT_pSLT
GCAGCGCCCCCAGGGGTGTTACTTAAAAACAGGCACAGCGACGCTAGCAGCGCCCCTGATATAATGCAATGTTTTTTATAAAAATAGTCAGTACTACCCCTGTATAGCGTCGTCATAGCGGTGCCGGCGACGTGCCTGCACCGCTATTTAATACATTAAAATAAATTAAATTACATTATTATGAACATAACTCACTGTTACTAAAAACAAATCAGAAAAATTTTAATTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAG
GCAGCGCCCCCAGGGGTGTTACTTAAAAACAGGCACAGCGACGCTAGCAGCGCCCCTGATATAATGCAATGTTTTTTATAAAAATAGTCAGTACTACCCCTGTATAGCGTCGTCATAGCGGTGCCGGCGACGTGCCTGCACCGCTATTTAATACATTAAAATAAATTAAATTACATTATTATGAACATAACTCACTGTTACTAAAAACAAATCAGAAAAATTTTAATTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 300 | GenBank | NP_490592 |
| Name | TraI_pSLT |
UniProt ID | Q93GL4 |
| Length | 1752 a.a. | PDB ID | |
| Note | putative relaxase | ||
Relaxase protein sequence
Download Length: 1752 a.a. Molecular weight: 191683.72 Da Isoelectric Point: 5.9311
>NP_490592.1 conjugative transfer: oriT nicking-unwinding (plasmid) [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MMSIAQVRSAGSAAGYYSDRDNYYVLGSMEERWAGKGAEQLGLQGTVDKEVFTRVLEGRLPDGADLSRQQ
DGGNKHRPGYDLTFSAPKSVSLMAMLAGDKRLTEAHNQAVDIAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVVNVTQHDGEWKTLSSDKVGKTGFIENVYANQIAFGKIYRAVLKEKVEA
LGYETEVVGKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVKMAEWMQT
LKDTGFDISAYRESADRRAEIQAAQPVPSQEQPDIQQAVTQAIAGLSDRKVQFTYTDVLARTVGMLPPEA
GVIEKARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSSDIMKQNRVTVHPEKSVPRTGSYSD
AVSVLAQDRPSLAIISGQGGAAGQRERVAELTMMAREQGREVQIIVADRRSQTNLKQDERLSGELITGRR
QLQEGMLFSPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKEAGVNSYRWQ
GRQQTPATVISEPDRNVRYARLAGDFVAAVKAGEESVAQVSGMREQAILAGMIRSELKTQGILGQQDTMM
TALSPVWLDSRNRYLRDMYREGMVMEQWNPEKRSHDRYVIDRVTAQSHSLTLRDAQGETQMVRISALDSS
WSLFRPEKMPVADGERLMVTGKIPGLRVSVGDRLQVSAVNDGVMTVTVPGRAEPASLPVGDSPFTALKLE
NGWVETPGHSVSDSAKVFASVTQMAMDNATLNSLARSGRDVRLYSSLDETRTAEKLSRHPSFTVVSEQIK
ARAGEVSLETAISRQKAGLHTPAQQAIHLALPVVESKNLAFSQVDLLTEAKSFAAEGTGFADLGREIDAQ
IKRGDLLYVDVAKGYGTDLLISRASYDAEKSILHHILEGKEAVTPLMERVPGELMETLTSGQRAATRMIL
ETKDRFTVVQGYAGVGKTTQFRAVMSAVNLLPESERPRVVGLGPTHRAVGEMRSAGVEAQTLASFLHDTQ
LQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAA
DVAIMKEIVRQTPELRDAVYSLINRDVNKALSGLENVKPVQVPRLKGAWAPENSVTEFSRLQERELAKAA
QEAEKKGEAFPDVPVTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIQDALAKPGEQQVTVPVL
TTANIRDGELRRLSTWEAHPGALALVDNVYHRIAGISKEDGLITLEDKAGNTRLISPREAAAEGVTLYNP
ETIRVGAGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGKQTRVVRPGQDRAEQHIDLAYAITAHS
AQGASETFAIALEGTEGGRKQMAGFESAYVALSRMKQHVQVYTDDRQGWVKAINSAEQKGTAHDVLEPKS
EREMMNAERLFSTARELRDVAAGRAVLRNAGLAQGDSRARFIAPGRKYPQPYVALPAFDRNGKSAGIWLN
PLTTDDGAGLRGFTGEGRVKGSEEAQFVALQGSRNGESLLAGSMQDGVRIARENPDSGVVVRIVGDGRPW
NPVAITGGRVWGDIPDSNVQPGAGNGEPVTAEILAQRQAEEAVRRETERRADEIVRKMAEDKSDLPEEKT
AQAVREIAGQEQDRMTPPEREPPLPESVLREPQRERETIREVARENRGRERLQQMEQEMVRDLQKEKTPG
GD
MMSIAQVRSAGSAAGYYSDRDNYYVLGSMEERWAGKGAEQLGLQGTVDKEVFTRVLEGRLPDGADLSRQQ
DGGNKHRPGYDLTFSAPKSVSLMAMLAGDKRLTEAHNQAVDIAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVVNVTQHDGEWKTLSSDKVGKTGFIENVYANQIAFGKIYRAVLKEKVEA
LGYETEVVGKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVKMAEWMQT
LKDTGFDISAYRESADRRAEIQAAQPVPSQEQPDIQQAVTQAIAGLSDRKVQFTYTDVLARTVGMLPPEA
GVIEKARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSSDIMKQNRVTVHPEKSVPRTGSYSD
AVSVLAQDRPSLAIISGQGGAAGQRERVAELTMMAREQGREVQIIVADRRSQTNLKQDERLSGELITGRR
QLQEGMLFSPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKEAGVNSYRWQ
GRQQTPATVISEPDRNVRYARLAGDFVAAVKAGEESVAQVSGMREQAILAGMIRSELKTQGILGQQDTMM
TALSPVWLDSRNRYLRDMYREGMVMEQWNPEKRSHDRYVIDRVTAQSHSLTLRDAQGETQMVRISALDSS
WSLFRPEKMPVADGERLMVTGKIPGLRVSVGDRLQVSAVNDGVMTVTVPGRAEPASLPVGDSPFTALKLE
NGWVETPGHSVSDSAKVFASVTQMAMDNATLNSLARSGRDVRLYSSLDETRTAEKLSRHPSFTVVSEQIK
ARAGEVSLETAISRQKAGLHTPAQQAIHLALPVVESKNLAFSQVDLLTEAKSFAAEGTGFADLGREIDAQ
IKRGDLLYVDVAKGYGTDLLISRASYDAEKSILHHILEGKEAVTPLMERVPGELMETLTSGQRAATRMIL
ETKDRFTVVQGYAGVGKTTQFRAVMSAVNLLPESERPRVVGLGPTHRAVGEMRSAGVEAQTLASFLHDTQ
LQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAA
DVAIMKEIVRQTPELRDAVYSLINRDVNKALSGLENVKPVQVPRLKGAWAPENSVTEFSRLQERELAKAA
QEAEKKGEAFPDVPVTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIQDALAKPGEQQVTVPVL
TTANIRDGELRRLSTWEAHPGALALVDNVYHRIAGISKEDGLITLEDKAGNTRLISPREAAAEGVTLYNP
ETIRVGAGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGKQTRVVRPGQDRAEQHIDLAYAITAHS
AQGASETFAIALEGTEGGRKQMAGFESAYVALSRMKQHVQVYTDDRQGWVKAINSAEQKGTAHDVLEPKS
EREMMNAERLFSTARELRDVAAGRAVLRNAGLAQGDSRARFIAPGRKYPQPYVALPAFDRNGKSAGIWLN
PLTTDDGAGLRGFTGEGRVKGSEEAQFVALQGSRNGESLLAGSMQDGVRIARENPDSGVVVRIVGDGRPW
NPVAITGGRVWGDIPDSNVQPGAGNGEPVTAEILAQRQAEEAVRRETERRADEIVRKMAEDKSDLPEEKT
AQAVREIAGQEQDRMTPPEREPPLPESVLREPQRERETIREVARENRGRERLQQMEQEMVRDLQKEKTPG
GD
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q93GL4 |
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 60123..86560
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PSLT068 | 55279..57276 | + | 1998 | NP_490556 | putative ParB-like nuclease domain | - |
| PSLT069 | 57316..57750 | + | 435 | NP_490557 | Plasmid SOS inhibition | - |
| PSLT070 | 57747..58484 | + | 738 | NP_490558 | Plasmid SOS inhibition | - |
| PSLT072 | 60123..60593 | - | 471 | NP_490559 | putative transglycosylase | virB1 |
| PSLT073 | 61008..61388 | + | 381 | NP_490560 | conjugative transfer: mating signal | - |
| PSLT075 | 61581..62267 | + | 687 | NP_490561 | conjugative transfer: regulation | - |
| PSLT076 | 62268..62588 | + | 321 | NP_490562 | conjugative transfer: oriT nicking | - |
| PSLT077 | 62630..62992 | + | 363 | NP_490563 | conjugative transfer: fimbrial subunit | - |
| PSLT078 | 63007..63318 | + | 312 | NP_490564 | conjugative transfer: assembly | traL |
| PSLT079 | 63340..63906 | + | 567 | NP_490565 | conjugative transfer: assembly | traE |
| PSLT080 | 63893..64633 | + | 741 | NP_490566 | conjugative transfer: assembly | traK |
| PSLT081 | 64633..66054 | + | 1422 | NP_490567 | conjugative transfer: assembly | traB |
| PSLT082 | 66044..66640 | + | 597 | NP_490568 | conjugative transfer | - |
| PSLT083 | 66618..66863 | + | 246 | NP_490569 | conjugative transfer protein | virb4 |
| PSLT084 | 66860..67375 | + | 516 | NP_490570 | conjugative transfer: assembly | traV |
| PSLT085 | 67510..67731 | + | 222 | NP_490571 | conjugative transfer | - |
| PSLT087 | 67724..68191 | + | 468 | NP_490572 | conjugative transfer protein | - |
| PSLT088 | 68587..71235 | + | 2649 | NP_490573 | conjugative transfer: assembly | virb4 |
| PSLT089 | 71210..71596 | + | 387 | NP_490574 | conjugative transfer | - |
| PSLT091 | 71593..72225 | + | 633 | NP_490575 | conjugative transfer: assembly | traW |
| PSLT092 | 72222..73214 | + | 993 | NP_490576 | conjugative transfer: assembly | traU |
| PSLT093 | 73233..73541 | + | 309 | NP_490577 | conjugative transfer protein | - |
| PSLT094 | 73526..74164 | + | 639 | NP_490578 | conjugative transfer: assembly | trbC |
| PSLT095 | 74617..76443 | + | 1827 | NP_490579 | conjugative transfer: aggregate stability | traN |
| PSLT096 | 76454..76669 | + | 216 | NP_490580 | conjugative transfer | - |
| PSLT097 | 76681..77430 | + | 750 | NP_490581 | conjugative transfer: assembly | traF |
| PSLT098 | 77644..77937 | + | 294 | NP_490582 | conjugative transfer: fimbrial synthesis | - |
| PSLT099 | 77924..78472 | + | 549 | NP_490583 | conjugative transfer | traF |
| PSLT100 | 78462..79844 | + | 1383 | NP_490584 | conjugative transfer: assembly | traH |
| PSLT101 | 79841..82663 | + | 2823 | NP_490585 | conjugative transfer: assembly abd aggregate stability | traG |
| PSLT102 | 82679..83173 | + | 495 | NP_490586 | conjugative transfer: surface exclusion | - |
| PSLT103 | 83209..83940 | + | 732 | NP_490587 | conjugative transfer: surface exclusion | - |
| PSLT104 | 84317..86560 | + | 2244 | NP_490588 | conjugative transfer: DNA transport | prgC |
| PSLT105 | 86560..87279 | + | 720 | NP_490589 | conjugative transfer | - |
| PSLT106 | 86569..86967 | - | 399 | NP_490590 | homologue of mvpA, Shigella flexneri | - |
| PSLT107 | 86967..87197 | - | 231 | NP_490591 | putative cytoplasmic protein | - |
Host bacterium
| ID | 691 | GenBank | NC_003277 |
| Plasmid name | pSLT | Incompatibility group | IncFII |
| Plasmid size | 93933 bp | Coordinate of oriT [Strand] | 60667..60925 [-] |
| Host baterium | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | Salmonella plasmid virulence |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |