Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100228
• Name: oriT_pSFD10
• Organism: Salmonella enterica subsp. enterica serovar 67:c:15
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_003079 (991..1076 [-], 86 nt)
• oriT length: 86 nt
• IRs (inverted repeats): _
• Location of nic site: 59..60
• Conserved sequence flanking the nic site: CTGG|CTTA
• Note: predicted by the oriTfinder

oriT sequence

• Length: 86 nt

>oriT_pSFD10
AGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACCATGCGGCATCAGTGCAGATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

MobA_pSFD10

• ID: 299
• GenBank: NP_203138
• Name: MobA_pSFD10
• UniProt ID: Q93LC3
• Length: 517 a.a.
• Note: putative relaxase

Relaxase protein sequence

• Length: 517 a.a.
• Molecular weight: 58216.86 Da
• Isoelectric Point: 6.4495

>NP_203138.1 mobilization protein MobA (plasmid) [Salmonella enterica]
MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSFAEKELPPGGR
EKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVN
HHYGLHDPNAPENRRTLTLPDNLPETKQALAESVTRGLEVLYHAGEIKGRQDVIQALTEVGLEVVRVTRS
SISIADPNGGRNIRLKGAFYEQYFTDGRGVREKAERESRIYRENAERRIQQARRICKRGCDLKRDENQRR
YSPVHNVDRGIAEKTPGRGERGDDAAPEGRVKAGREYGRDVTGDSLSPVYREWRDALVSWREDTDEPGRN
QGTGRDIAETEREDMGRGVCSGREKEISGPSVREISRGDALSGEGLDTTDGVIRNDRAGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGTALERADRTLEPVIQRELEIREERLIQE
REHALSLERERQLEIQERTLDGPSLSL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (57-236)

Protein structure

• AlphaFold DB: Q93LC3

Host bacterium

• ID: 690
• GenBank: NC_003079
• Plasmid name: pSFD10
• Incompatibility group: ColRNAI
• Plasmid size: 4091 bp
• Coordinate of oriT [Strand]: 991..1076 [-]
• Host baterium: Salmonella enterica subsp. enterica serovar 67:c:15

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -