Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100223
Name   oriT_pMD136 in_silico
Organism   Pediococcus pentosaceus
Sequence Completeness      incomplete
NCBI accession of oriT (coordinates [strand])   NC_002126 (19376..19438 [+], 63 nt)
oriT length   63 nt
IRs (inverted repeats)      1..31, 33..63  (GTAAATTGATTGATTAAGCTCGTGAAACCCT..AGGGTTTCACGAGCTTAATCAATCAATTTAC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   predicted by the oriTfinder

  oriT sequence  


Download         Length: 63 nt

>oriT_pMD136
GTAAATTGATTGATTAAGCTCGTGAAACCCTTAGGGTTTCACGAGCTTAATCAATCAATTTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   294 GenBank   NP_052581
Name   Orf2_pMD136 insolico UniProt ID   Q9WW30
Length   386 a.a. PDB ID   
Note   putative relaxase

  Relaxase protein sequence


Download         Length: 386 a.a.        Molecular weight: 44687.23 Da        Isoelectric Point: 10.0930

>NP_052581.1 Orf2 (plasmid) [Pediococcus pentosaceus]
MATTHIKRSDSASRLVNYAEKRAVLKDGLNLDVDYAKSQLKQVREVYGNQGKTQAYASRISFSPKELDPT
NENDQQKALEIAKEVYQKTYPNQQVALYEHADTNALHVHAVIGAIDLETGRKMHGNWQEYREKLVHNTDE
ISQEHGLEVTQVDPNRYEKRTMAEIKMQAKGQPTWKDQIRQAVDKTMANRLVTDFQTFRDDLKQKTVDVW
ERGKDLTYQLTGTNYKARGNKLGTAYEKGEIYHELERRTRNHTKTRNPDTAKPDRTQPNLERNQELSGHA
NSITEQTTDRIDRTAPTDRASQSTGQSGFSNDLEQFRKQQRDQQTTINRDAQRRARTVQRANTQDKSKDN
RSLKTDKQRPKEQQRDVEKSQQQSKSNRSRNDGPSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-248)


  Protein structure


Source ID Structure
AlphaFold DB Q9WW30


Host bacterium

ID   685 GenBank   NC_002126
Plasmid name   pMD136 Incompatibility group   _
Plasmid size   19533 bp Coordinate of oriT [Strand]   19376..19438 [+]
Host baterium   Pediococcus pentosaceus

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -