Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100221 |
| Name | oriT_pNoc614  |
| Organism | Nocardioides sp. JS614 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | AY772007 ( , 510 nt) |
| oriT length | 510 nt |
| IRs (inverted repeats) | IR1: 179..183, 185..189 (GGGCG..CGCCC) IR2: 225..237, 245..257 (GTTGGATTCGGCG..CGCCGAATCCACC) |
| Location of nic site | 155..156 |
| Conserved sequence flanking the nic site |
TACCCGCG|CCCT |
| Note | The putative JS614 oriT region contains a sequence (TACCCGCG|CCCT) very similar to the consensus oriT core sequence identified in RSF1010, pTF1, and Ti plasmids (TAAGTGCG|CCCT)(Cook and Farrand 1992). |
oriT sequence
Download Length: 510 nt
>oriT_pNoc614
TTGAGGCCGCGGCCGCGAAGTCGCCGGCGGAACGCTGGGCCCCGCTGGCCCGCGAGGTCGACCCGCGCCTGGCGGAGCAGGACGACTGGCCCGCCACCGCCGCGATGCTGCAACAGGCCCACGAGCAAGGCCACGACGTCAGCGCCGCTACCCGCGCCCTGGTCGCAGCAGCACCCCTGGGCGACGCCCCCGCCCGCGAACTGCGCTACCGGCTGGTTCGGCAGGTTGGATTCGGCGAACACGACGCCGAATCCACCGACCGTGACGCCCCGAAGCGCCGGCGCGACGCGGGTACTGGACATGAGCTCGGCTCTTCGAACCCAGTGCACCGCAGCCGCCGCCGGTAGGCCTGGGCACAGGTTGAGACCTACAACGAGAGGGCTGTCGGACACTCAACGCGAGGTACCCGCGTGCAGACCTGCCACATTGCGGCCCACTGTGGGGCAGCGTCATGGCGCTCAAACGGGCGGGCTCCTTGTCCAGAGGTCTTCAAAGCGCCGGACTGGGCCG
TTGAGGCCGCGGCCGCGAAGTCGCCGGCGGAACGCTGGGCCCCGCTGGCCCGCGAGGTCGACCCGCGCCTGGCGGAGCAGGACGACTGGCCCGCCACCGCCGCGATGCTGCAACAGGCCCACGAGCAAGGCCACGACGTCAGCGCCGCTACCCGCGCCCTGGTCGCAGCAGCACCCCTGGGCGACGCCCCCGCCCGCGAACTGCGCTACCGGCTGGTTCGGCAGGTTGGATTCGGCGAACACGACGCCGAATCCACCGACCGTGACGCCCCGAAGCGCCGGCGCGACGCGGGTACTGGACATGAGCTCGGCTCTTCGAACCCAGTGCACCGCAGCCGCCGCCGGTAGGCCTGGGCACAGGTTGAGACCTACAACGAGAGGGCTGTCGGACACTCAACGCGAGGTACCCGCGTGCAGACCTGCCACATTGCGGCCCACTGTGGGGCAGCGTCATGGCGCTCAAACGGGCGGGCTCCTTGTCCAGAGGTCTTCAAAGCGCCGGACTGGGCCG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Mattes TE et al. (2005) Physiological and molecular genetic analyses of vinyl chloride and ethene biodegradation in Nocardioides sp. strain JS614. Arch Microbiol. 183(2):95-106. [PMID:15599705]
Relaxase
| ID | 214 | GenBank | AAV52093 |
| Name | TraA_pNoc614 |
UniProt ID | Q5U9L8 |
| Length | 1818 a.a. | PDB ID | |
| Note | putative TraA conjugation protein | ||
Relaxase protein sequence
Download Length: 1818 a.a. Molecular weight: 195865.85 Da Isoelectric Point: 5.0409
>AAV52093.1 putative TraA conjugation protein [Nocardioides sp. JS614]
MPVTMSLHKLTAGSGYDYLTRQVAAMDATDKGHTGLASYYTEKGETPGVWVGSGMEGIEGLDAGDVVTAD
HMQNLFGAGHHPLATARTKELDLRVGRAGVGSPTAADYKAAARLGTPFKVYENDVSAFRVEVAKRLAAVN
EAAGLPGDWPVPAEERARIRTEIGRDFFRAEHGREPADARELAATIAKHSRPKTNAVAGYDLTFSPVKSV
STLWALADPKTAAVIEGAHQSAIKDALNFIETKALFTRQGTNGIRQVNVRGLLATAFTHRDSRAGDPDLH
THVAVANKVQTLDGKWLAIDGRLLYKAKVSASETYNTALERHLGDALGGTVRGTAESRRPEAAGARDRRL
SYPDLNHRFLQAPRSASKPAARSWPPSSRRPTAARRHRIETLQLAQRATLETREAKHEPRSLNDQREAWD
REAVEVLGSPRGLQQMLHGTLNPTAAGTAPVADAAWFAKTGDRIVTTMEGGRATWQYWHVYAEAQRQVRA
ANVPTDQVSTVVGLLVSEVLDGRSVSITRPFDEISEPPELRKLDGASVYTVGGVHLFTSSKVLAAEERLV
EAAGRRDGYAVDEQNVALALLESEANGVTLNAGQATLVKEMATSGARLQLAIAPAGSGKTTAMRALAQAW
TDGGGSVIGLAPSAAAADALRVSMGSQIDTQTDTLAKLTHSLEQSQVTGAEVPDWVAGIGPQTLVVIDEA
GMADTLSLDIAVQHVLERGGSVRLVGDDQQLAAIGAGGVLRDIRATHGALQLTELVRFTDPAEGAASLAL
REGKGEALGFYLDRDRVHVGDLATMTEDVFAAWQADRAAGLDAIMLAPTRELVSELNQQARAHRLEGADP
ASVGPARRLADGNEASIGELIITRTNDRKLRTSATDWVKNGDRWTVLGLHEGGDLTVQHTQHGRTVRLPR
EYVETSTELGYACTVHTAQGVTTDTMHGLATGNESRQQLYTMLTRGKSGNHIYLQVVGDGDPHSVIHPTL
VRPLTPTDILESMLARDDAQRSATSLMREQADPATQLGEAAQRYLDSLYVAAENHLRNTAGPDGSNVVEA
LDSTVDQLLPGLADEAAWPTLRAHLLLLGAAGENPVEAMRAAASDRELVSAEDRAAVLDWRLDASGMRNV
GTGPLPWIPGVPERLADDPHWGAYLDQRAHLVDQLADEVRGRAAAQTSLPTWAQNGIRPDTDTVADVEVW
RAAMQVPLDDRRPTGAPQLQKASSTYQRRLNRRITGDHTPALTEWRQLLYSLAPQVRGDEFTPLLAERLA
AMSRAGVEAHQLLRTATAADTGAGPLPDEHAAAALWWRMARHLSPAVAAQVGDGSHGEGVTTSWAAQLPE
LLSDERSARIQASAWWPALVSNIDHGLQRGWQLEALLSAGSALPSDDIDECQALVWRTSIALEPIPDEHE
HDDHFDEPPVDMWDGVEPDPATLVDHPDDTEWPLPHDLEADGDHLVDHPEPDEAIDQVDVAPAAADNVDE
QQFVEGDLTLAAYIRDLGGTPLEPTAADIRLMYQRADEWYSFPVTRERMIEINEITQTFFESRFTDSWGR
DYLTGRFGIDLAGHEHFRPGQAPAGWTNLVDHLRHRGVSGAEMLATGVATTASTGRLIDRFRDRVMFPVV
HNGEVLGFVGRRRPELTDADQANGRGGPKYLNTADTPLFHKGAQLYGVVDELLAEGAVPVIVEGPMDAIA
VTLASAARYVGVAPLGTSLTDEQASQLGGIGRDPIVATDADLAGEVAAERDFWMLTPHGLDPGYARFPEG
LDPADLLAQRGPAALTASLASGQTLGDQLLSERLDNLAPEQARVAAMRVLAARPSRAWRRAPTWCAPD
MPVTMSLHKLTAGSGYDYLTRQVAAMDATDKGHTGLASYYTEKGETPGVWVGSGMEGIEGLDAGDVVTAD
HMQNLFGAGHHPLATARTKELDLRVGRAGVGSPTAADYKAAARLGTPFKVYENDVSAFRVEVAKRLAAVN
EAAGLPGDWPVPAEERARIRTEIGRDFFRAEHGREPADARELAATIAKHSRPKTNAVAGYDLTFSPVKSV
STLWALADPKTAAVIEGAHQSAIKDALNFIETKALFTRQGTNGIRQVNVRGLLATAFTHRDSRAGDPDLH
THVAVANKVQTLDGKWLAIDGRLLYKAKVSASETYNTALERHLGDALGGTVRGTAESRRPEAAGARDRRL
SYPDLNHRFLQAPRSASKPAARSWPPSSRRPTAARRHRIETLQLAQRATLETREAKHEPRSLNDQREAWD
REAVEVLGSPRGLQQMLHGTLNPTAAGTAPVADAAWFAKTGDRIVTTMEGGRATWQYWHVYAEAQRQVRA
ANVPTDQVSTVVGLLVSEVLDGRSVSITRPFDEISEPPELRKLDGASVYTVGGVHLFTSSKVLAAEERLV
EAAGRRDGYAVDEQNVALALLESEANGVTLNAGQATLVKEMATSGARLQLAIAPAGSGKTTAMRALAQAW
TDGGGSVIGLAPSAAAADALRVSMGSQIDTQTDTLAKLTHSLEQSQVTGAEVPDWVAGIGPQTLVVIDEA
GMADTLSLDIAVQHVLERGGSVRLVGDDQQLAAIGAGGVLRDIRATHGALQLTELVRFTDPAEGAASLAL
REGKGEALGFYLDRDRVHVGDLATMTEDVFAAWQADRAAGLDAIMLAPTRELVSELNQQARAHRLEGADP
ASVGPARRLADGNEASIGELIITRTNDRKLRTSATDWVKNGDRWTVLGLHEGGDLTVQHTQHGRTVRLPR
EYVETSTELGYACTVHTAQGVTTDTMHGLATGNESRQQLYTMLTRGKSGNHIYLQVVGDGDPHSVIHPTL
VRPLTPTDILESMLARDDAQRSATSLMREQADPATQLGEAAQRYLDSLYVAAENHLRNTAGPDGSNVVEA
LDSTVDQLLPGLADEAAWPTLRAHLLLLGAAGENPVEAMRAAASDRELVSAEDRAAVLDWRLDASGMRNV
GTGPLPWIPGVPERLADDPHWGAYLDQRAHLVDQLADEVRGRAAAQTSLPTWAQNGIRPDTDTVADVEVW
RAAMQVPLDDRRPTGAPQLQKASSTYQRRLNRRITGDHTPALTEWRQLLYSLAPQVRGDEFTPLLAERLA
AMSRAGVEAHQLLRTATAADTGAGPLPDEHAAAALWWRMARHLSPAVAAQVGDGSHGEGVTTSWAAQLPE
LLSDERSARIQASAWWPALVSNIDHGLQRGWQLEALLSAGSALPSDDIDECQALVWRTSIALEPIPDEHE
HDDHFDEPPVDMWDGVEPDPATLVDHPDDTEWPLPHDLEADGDHLVDHPEPDEAIDQVDVAPAAADNVDE
QQFVEGDLTLAAYIRDLGGTPLEPTAADIRLMYQRADEWYSFPVTRERMIEINEITQTFFESRFTDSWGR
DYLTGRFGIDLAGHEHFRPGQAPAGWTNLVDHLRHRGVSGAEMLATGVATTASTGRLIDRFRDRVMFPVV
HNGEVLGFVGRRRPELTDADQANGRGGPKYLNTADTPLFHKGAQLYGVVDELLAEGAVPVIVEGPMDAIA
VTLASAARYVGVAPLGTSLTDEQASQLGGIGRDPIVATDADLAGEVAAERDFWMLTPHGLDPGYARFPEG
LDPADLLAQRGPAALTASLASGQTLGDQLLSERLDNLAPEQARVAAMRVLAARPSRAWRRAPTWCAPD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Reference
[1] Mattes TE et al. (2005) Physiological and molecular genetic analyses of vinyl chloride and ethene biodegradation in Nocardioides sp. strain JS614. Arch Microbiol. 183(2):95-106. [PMID:15599705]
Host bacterium
| ID | 683 | GenBank | AY772007 |
| Plasmid name | pNoc614 | Incompatibility group | _ |
| Plasmid size | 34814 bp | Coordinate of oriT [Strand] | _ |
| Host baterium | Nocardioides sp. JS614 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | utilizing vinyl chloride and ethene |
| Symbiosis gene | - |
| Anti-CRISPR | - |