Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100216
Name   oriT_pIE1130 in_silico
Organism   Uncultured eubacterium
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   AJ271879 (813..849 [-], 37 nt)
oriT length   37 nt
IRs (inverted repeats)      3..9, 13..19  (GTTTCTT..AGAAAC)
Location of nic site      30..31
Conserved sequence flanking the
  nic site    		  TAAGTGCG|CCCT
Note   similar to the oriT of IncQ plasmid RSF101

  oriT sequence  


Download         Length: 37 nt

>oriT_pIE1130
CAGTTTCTCGAAGAGAAACCGGTAAGTGCGCCCTCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Smalla K et al. (2000) Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids. Appl Environ Microbiol. 66(11):4854-62. [PMID:11055935]


Relaxase

ID   209 GenBank   CAB75594
Name   MobA_pIE1130 insolico UniProt ID   Q9L3P4
Length   703 a.a. PDB ID   
Note   relaxase

  Relaxase protein sequence


Download         Length: 703 a.a.        Molecular weight: 78483.58 Da        Isoelectric Point: 8.7777

>CAB75594.1 putative mobilization protein (plasmid) [uncultured bacterium]
MAIYHLTAKTGSRNGGQSAKAKADYIQREGRYSRDRDEVLHTQSGHLPEWAERPADYWDAADLYERANGR
LFKEVEFALPVELTLDQQRELVDEFARHLTEGERLPYTLAIHAGKGENPHCHLMISERKNDGIERPADQW
FKRYNAKQPERGGAQKSESLKPKGWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGIHLGP
NVVEMEGRGIRTERADTALAIDTANGQIIDLQEYREVIEHERDRQSEEVQRHQRVSGADRTAGPEHGDTG
RRGPAGHEPDSAGQRGAGSGVAESPTPDRGGMGGAGQGVAGGSRRGEQRRAERPERRPWLDMEVVGRGLD
RFRDAYSGAADRIMALAGAANHRTTGGYMAGFQAQVKGDRTAQAIARQLKAMGCDRYDIGIRDAASGKMM
NREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALIVETSPKNY
QAWVKVAQDAPAGHRGVIARKLAREYDADPASADSRHYGRLAGFTNRKDKHTTRTGYQPWVLLRESKGKT
ATAGPELMQQAGQVLDSIKRQQERTARLAEITAPRSVRRYRRSAVDDYRSEMAGLVKRFGDDLSKCDFIA
AMKLASKGREPDEIAKAMAEASPAIMERKAGHEADYIKRTVQKVMELPQVQEARAELAKQTQKQRSRGPD
LSM

  Protein domains


Predicted by InterproScan.

RepB DNA-primase from phage plasmid (RepB_primase)

(415-639)

MobA/MobL family (MobA_MobL)

(23-217)


  Protein structure


Source ID Structure
AlphaFold DB Q9L3P4


Auxiliary protein

ID   294 GenBank   CAB75595
Name   MobB_pIE1130 insolico UniProt ID   Q9L3P3
Length   136 a.a. PDB ID   _
Note   putative role in conjugative DNA replication

  Auxiliary protein sequence


Download         Length: 136 a.a.        Molecular weight: 14981.28 Da        Isoelectric Point: 6.3803

>CAB75595.1 putative mobilization protein (plasmid) [uncultured bacterium]
MSAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQASEAQAAEWLKAQRQTGAAWVELAKEL
REVAAEVSSAAQSARSAARGWTWKLWAGVLIASVTPILALLIASWLWLEPQIIEQQGGIWLVFKLK

  Protein domains


Predicted by InterproScan.

Mobilization protein B (Mobilization_B)

(1-136)


  Protein structure


Source ID Structure
AlphaFold DB Q9L3P3

ID   295 GenBank   CAB75593
Name   MobC_pIE1130 insolico UniProt ID   Q9L3P5
Length   90 a.a. PDB ID   _
Note   putative role in conjugative DNA replication

  Auxiliary protein sequence


Download         Length: 90 a.a.        Molecular weight: 10547.96 Da        Isoelectric Point: 10.1923

>CAB75593.1 putative mobilization protein (plasmid) [uncultured bacterium]
MSKAAERLAKLEEQRARINAEIQRVRAREQQQKRKEDTRRKVLVGAWILGKVESGEWPEQRLLDGLDSYL
ERDHDRALFGLPPRQKDEPG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB Q9L3P5


Host bacterium

ID   209 GenBank   AJ271879
Plasmid name   pIE1130 Incompatibility group   IncQ1
Plasmid size   10687 bp Coordinate of oriT [Strand]   813..849 [-]
Host baterium   Uncultured eubacterium

Cargo genes

Drug resistance gene   resistance to sulfonamide, chloramphenicol, kanamycin and streptomycin
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -