Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100215
Name   oriT_pIE1115 in_silico
Organism   Uncultured eubacterium pIE1115
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   NC_002524 (371..407 [-], 37 nt)
oriT length   37 nt
IRs (inverted repeats)      3..8, 14..19  (GTTTCTT..AGAAAC)
Location of nic site      30..31
Conserved sequence flanking the
  nic site    		  TAAGTGCG|CCCT
Note   similar to the oriT of IncQ plasmid RSF101

  oriT sequence  


Download         Length: 37 nt

>oriT_pIE1115
CAGTTTCTTGAAGAGAAACCGGTAAGTGCGCCCTCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Smalla K et al. (2000) Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids. Appl Environ Microbiol. 66(11):4854-62. [PMID:11055935]


Relaxase

ID   208 GenBank   NP_065281
Name   MobA_pIE1115 insolico UniProt ID   Q9FBF9
Length   703 a.a. PDB ID   
Note   relaxase

  Relaxase protein sequence


Download         Length: 703 a.a.        Molecular weight: 78751.69 Da        Isoelectric Point: 7.6999

>NP_065281.1 putative mobilization protein (plasmid) [uncultured eubacterium pIE1115]
MAIYHLTAKTGSRNGGQSAKAKADYIQREGRYSRDREEVLHTQSGHLPEWAERPADYWDGADLYERANGR
LFKEVEFALPVELTLDQQRELVDEFARHLTEGERLPYTLAIHAGNGENPHCHLMISERKNDGIERPADQW
FKRYNGKQPERGGAQKSESLKPKAWLEQTREAWSHYANRALEQAGHEARIDHRTLEAQGIERLPGIHLGP
NVVEMESRGIRTERADIALAIDTANGQIIDLQEYREVIEHERDRQSEEIQRDQRVSGRDRAAGPEHGDTG
GRSPAADRRGTAGQRGAGRELDEPATASHERMAAGSKGHEGSRRGTGQGRPDGPERRPWLDMEVVGRGLD
RFRDAYSGAADRIMALAGAANHRATGEHMADFQAQVKGDRTAQAIARQLKAMGCDRYDIGIRDAASGKMM
NREWTPQEVQQNAAWLKRMNAQGNDIYIRPAEQARHGLVLVDDLSIDDLDAMKQEGREPAAIIETSPKNY
QAWVKVAQDAPAGHRGVIARKLAREYDADPASADSRHYGRLAGFTNRKDKYTSRTGYQPWVLCRESSGKS
ATAGPELMQQAGQVLDSIERRQERTARLAEITAPQSVRRYRRSVVDDYRSEMAGLVKRYGDDLSKCDFIA
AMKLASKGREPDEIGKAMAEASPAIMERKAGHEADYIKRTVQKVMELPQVQEARAELAKQAQKQRSRGPD
LSM

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(22-218)

RepB DNA-primase from phage plasmid (RepB_primase)

(415-640)


  Protein structure


Source ID Structure
AlphaFold DB Q9FBF9


Auxiliary protein

ID   292 GenBank   NP_065282
Name   MobB_pIE1115 insolico UniProt ID   Q7AZ28
Length   136 a.a. PDB ID   _
Note   putative role in conjugative DNA replication

  Auxiliary protein sequence


Download         Length: 136 a.a.        Molecular weight: 15085.39 Da        Isoelectric Point: 10.1732

>NP_065282.1 putative mobilization protein (plasmid) [uncultured eubacterium pIE1115]
MSAIDRVRKSRGINELAAEIEPLAQSMATLADEARQRIAEVQQASEEQAASWTSQQQQAMSAWRQAAKDM
RAAAGELAKAGQTARSAARGWTWRLWAGVLIASVMPILALLIASWLWLEPQIIEQQGSIWLIFKLK

  Protein domains


Predicted by InterproScan.

Mobilization protein B (Mobilization_B)

(1-136)


  Protein structure


Source ID Structure
AlphaFold DB Q7AZ28

ID   293 GenBank   NP_065280
Name   MobC_pIE1115 insolico UniProt ID   Q9FBG0
Length   95 a.a. PDB ID   _
Note   putative role in conjugative DNA replication

  Auxiliary protein sequence


Download         Length: 95 a.a.        Molecular weight: 10982.49 Da        Isoelectric Point: 10.1923

>NP_065280.1 putative mobilization protein (plasmid) [uncultured eubacterium pIE1115]
MSKAAERLAKLEEQRARINAEIQRVRAREQQQKRKEDTRRKVLVGAWILGKVESGEWPEQRLLDGLDSYL
ERDHDRALFGLPPKGSFAGEGEILR

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB Q9FBG0


Host bacterium

ID   208 GenBank   NC_002524
Plasmid name   pIE1115 Incompatibility group   IncQ1
Plasmid size   10687 bp Coordinate of oriT [Strand]   371..407 [-]
Host baterium   Uncultured eubacterium pIE1115

Cargo genes

Drug resistance gene   resistance to and sulfonamide
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -