Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100214
Name   oriT_pLS55 in_silico
Organism   Latilactobacillus sakei
Sequence Completeness      incomplete
NCBI accession of oriT (coordinates [strand])   NC_010375 (2038..2072 [+], 35 nt)
oriT length   35 nt
IRs (inverted repeats)      6..9, 10..13  (AGAA..TTCT)
Location of nic site      24..25
Conserved sequence flanking the
  nic site    		  TACCGTG|TTAT
Note   _

  oriT sequence  


Download         Length: 35 nt

>oriT_pLS55
CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Alippi AM et al. (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]


Relaxase

ID   207 GenBank   YP_001691307
Name   Mob_pLS55 insolico UniProt ID   B0YMX1
Length   439 a.a. PDB ID   
Note   relaxase

  Relaxase protein sequence


Download         Length: 439 a.a.        Molecular weight: 52396.34 Da        Isoelectric Point: 6.3441

>YP_001691307.1 Mob (plasmid) [Lactobacillus sakei]
MEVYEMAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDERLSEVYCMNR
KDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSANVHNDETTPHMHFDFVPVVWD
EKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQEGILNDKTIGIENIHSLKKYSAEIEQQKEEM
ANEFKSFKYPQKVLKSIEREVEKKTVGVFNKKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEI
KNLKVNVKNFEEDNEHLRYRNIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLH
NLENDRMPKNKEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKEL
SQQRQQQKKTKSRSSGMEL

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(1-173)


  Protein structure


Source ID Structure
AlphaFold DB B0YMX1


Host bacterium

ID   207 GenBank   NC_010375
Plasmid name   pLS55 Incompatibility group   _
Plasmid size   5031 bp Coordinate of oriT [Strand]   2038..2072 [+]
Host baterium   Latilactobacillus sakei

Cargo genes

Drug resistance gene   resistance to tetracycline (tetL)
Virulence gene   _
Metal resistance gene   _
Degradation gene   _
Symbiosis gene   -
Anti-CRISPR   -