Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 100211 |
| Name | oriT_pRS01  |
| Organism | Lactococcus lactis subsp. lactis |
| Sequence Completeness | core |
| NCBI accession of oriT (coordinates [strand]) | U50902 (5497..5536 [+], 40 nt) |
| oriT length | 40 nt |
| IRs (inverted repeats) | 4..11, 16..23 (GTAAGATG..CATCTTAC) |
| Location of nic site | 31..32 |
| Conserved sequence flanking the nic site |
CTTG|CA |
| Note | _ |
oriT sequence
Download Length: 40 nt
>oriT_pRS01
TCCGTAAGATGCTATCATCTTACTATGCTTGCAAAAGGTC
TCCGTAAGATGCTATCATCTTACTATGCTTGCAAAAGGTC
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Novikova O et al. (2014) Interaction between conjugative and retrotransposable elements in horizontal gene transfer. PLoS Genet. 10(12):e1004853. [PMID:25474706]
Relaxase
| ID | 204 | GenBank | AAB06502 |
| Name | LtrB_pRS01 |
UniProt ID | Q48722 |
| Length | 563 a.a. | PDB ID | |
| Note | relaxase | ||
Relaxase protein sequence
Download Length: 563 a.a. Molecular weight: 64870.41 Da Isoelectric Point: 6.5160
>AAB06502.2 relaxase (plasmid) [Lactococcus lactis subsp. lactis]
MVYTKHIIVHKLKHLRQAKDYVENAEKTLVNESNEDHLTNLFPYISNPDKTMSKQLVSGHGITNVYDAAN
EFIATKKLKALSKGTDFNFDPQTGKVRFNVESLEKNNAVLGHHLIQSFSPDDNLTPEQIHEIGRQTILEF
TGGEYEFVIATHVDREHIHNHIIFNSTNLYTGKQFDWKVIPKEKTKSGKAYDVTKNNFEKVSDKIASRYG
AKIIEKSPGNSHLKYTKWQTQSIYKSQIKQRLDYLLEMSSDIEDFKRKATALNLSFDFSGKWTTYRLLDE
PQMKNTRGRNLDKNRPEKYNLESIIERLETNELSLTVDEVVERYEEKVDVVKQDFDYQVTVEKGQIDHMT
SKGFYLNVDFGIADRGQIFIGGYKVDQLENRDCVLYLKKNETFRLLSEKEASFTKYLTGHDLAKQLGLYN
GTVPLKKEPVISTINQLVDAINFLAEHGVTEGTQFNNMESQLMSALGEAEEKLYVIDNKIMELTKIAKLL
IEKESDHSQAVINELENLGVGPSIKYQDIHQELQSEKMSRKILKNKFEQTVDEINTFNEIRVTTLEENKG
KIL
MVYTKHIIVHKLKHLRQAKDYVENAEKTLVNESNEDHLTNLFPYISNPDKTMSKQLVSGHGITNVYDAAN
EFIATKKLKALSKGTDFNFDPQTGKVRFNVESLEKNNAVLGHHLIQSFSPDDNLTPEQIHEIGRQTILEF
TGGEYEFVIATHVDREHIHNHIIFNSTNLYTGKQFDWKVIPKEKTKSGKAYDVTKNNFEKVSDKIASRYG
AKIIEKSPGNSHLKYTKWQTQSIYKSQIKQRLDYLLEMSSDIEDFKRKATALNLSFDFSGKWTTYRLLDE
PQMKNTRGRNLDKNRPEKYNLESIIERLETNELSLTVDEVVERYEEKVDVVKQDFDYQVTVEKGQIDHMT
SKGFYLNVDFGIADRGQIFIGGYKVDQLENRDCVLYLKKNETFRLLSEKEASFTKYLTGHDLAKQLGLYN
GTVPLKKEPVISTINQLVDAINFLAEHGVTEGTQFNNMESQLMSALGEAEEKLYVIDNKIMELTKIAKLL
IEKESDHSQAVINELENLGVGPSIKYQDIHQELQSEKMSRKILKNKFEQTVDEINTFNEIRVTTLEENKG
KIL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q48722 |
Reference
[1] Novikova O et al. (2014) Interaction between conjugative and retrotransposable elements in horizontal gene transfer. PLoS Genet. 10(12):e1004853. [PMID:25474706]
[2] Mills DA et al. (1996) Splicing of a group II intron involved in the conjugative transfer of pRS01 in lactococci. J Bacteriol. 178(12):3531-8. [PMID:8655550]
Host bacterium
| ID | 204 | GenBank | U50902 |
| Plasmid name | pRS01 | Incompatibility group | _ |
| Plasmid size | 12851 bp | Coordinate of oriT [Strand] | 5497..5536 [+] |
| Host baterium | Lactococcus lactis subsp. lactis |
Cargo genes
| Drug resistance gene | _ |
| Virulence gene | _ |
| Metal resistance gene | _ |
| Degradation gene | _ |
| Symbiosis gene | - |
| Anti-CRISPR | - |