Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100207
Name   oriT_pAD1 experimental
Organism   Enterococcus faecalis
Sequence Completeness      incomplete
NCBI accession of oriT (coordinates [strand])   AH011360 ( , 13 nt)
oriT length   13 nt
IRs (inverted repeats)     _
Location of nic site      6..7
Conserved sequence flanking the
  nic site    		  _
Note   CloDF13 familiy

  oriT sequence  


Download         Length: 13 nt

>oriT_pAD1
GAAAATGGTCAGG

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Francia MV et al. (2004) A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531]
[2] Francia MV et al. (2002) Transfer origins in the conjugative Enterococcus faecalis plasmids pAD1 and pAM373: identification of the pAD1 nic site, a specific relaxase and a possible TraG-like protein. Mol Microbiol. 45(2):375-95. [PMID:12123451]
[3] Francia MV et al. (2001) Completion of the nucleotide sequence of the Enterococcus faecalis conjugative virulence plasmid pAD1 and identification of a second transfer origin. Plasmid. 46(2):117-27. [PMID:11591137]


Relaxase

ID   200 GenBank   AAL59457
Name   Orf57_pAD1 insolico UniProt ID   Q8VT37
Length   216 a.a. PDB ID   
Note   Replication-relaxation; pfam13814

  Relaxase protein sequence


Download         Length: 216 a.a.        Molecular weight: 26040.98 Da        Isoelectric Point: 8.3546

>AAL59457.1 unknown (plasmid) [Enterococcus faecalis]
MAKKSSVYGNLKKLKEKNLVECSQIGSTKFYYLTKEGHNYIGGYYTLPKVPEYNLQHHLQINDYLIKMLE
LCNNHPHLKAVVSERRKVYEVKDEKKNQKGVKYFVPDFIFMFLDSIGREVEWQFEIELTLKTKRRYSQGV
FPKYIKHLKNYEDARLIYVTPSSIIKEELDMFKEYFIDKEGDEYKEVFDRLHVFSAEEFESELKRLLEKD
KFINWE

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB Q8VT37

  Reference


[1] Francia MV et al. (2004) A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531]
[2] Francia MV et al. (2002) Transfer origins in the conjugative Enterococcus faecalis plasmids pAD1 and pAM373: identification of the pAD1 nic site, a specific relaxase and a possible TraG-like protein. Mol Microbiol. 45(2):375-95. [PMID:12123451]
[3] Francia MV et al. (2001) Completion of the nucleotide sequence of the Enterococcus faecalis conjugative virulence plasmid pAD1 and identification of a second transfer origin. Plasmid. 46(2):117-27. [PMID:11591137]


T4CP

ID   200 GenBank   AAL59453
Name   Orf53_pAD1 insolico UniProt ID   Q8VT41
Length   747 a.a. PDB ID   _
Note   conjugative coupling factor TraD, SXT/TOL subfamily; TIGR03743

  T4CP protein sequence


Download         Length: 747 a.a.        Molecular weight: 85562.94 Da        Isoelectric Point: 6.3682

>AAL59453.1 unknown (plasmid) [Enterococcus faecalis]
MFNNGLLQPKKPPIGQEQQKMTRYHQLRLVFGLVGVLLYPFAILGSIPLLIGLAVDKRDKAANVFDMDYE
SFLKRNSIVFLSFSAVLFVINAFAFILWIPRGYLSAYLLFPLNLLHTALRFNWETIVALLIGSSGMGAIF
LAFSSFVAKRKVISKEDERKKITESKAYKDREKNKFKESQRFTDEQEEAYEEAVETVDIDKYKELSNQLL
LGTSEFGLPYIINFSEFNQHVLVPATTGSGKTTLLQLLVQHAVKFNFPVILIDGKGARDTLESMREIAKF
YDKEVHAFTDDGDMRYNPVEHGNDISIRDKLVSLAETESVFYSGAAKALLQVTIQLLDEFKGAKVTLSGD
TRTTETVERSLPFVQRFLLPRNVLHLFADAILQNNPKLFEIEVEKKIQQPKKKSAKERSEILPDSDLDKE
EKEEDSQIRNSKFRNISQLGIAQKETETVVLNPETLDLDSYYLLLKRNLRYLPTDKETGENIKQKLFERL
FVRYEHKDSPFYLYATSEALQTNINMLLDSELGKLFDTKNAKNVLDVQEIVNQRKLVYVSFNGLIYKEYI
RTLAQMLVGDVNYFASEMYRKNVKREVLVIFDEPASYLNETFIDMVNKGRGAGVYGIFTPQTMADIAKLG
DKLMEQLVGNVNTLFIGKTNEKGEAEYWSETMGTYQDIDVTSVTEQEDGYSDVGKSDWSGDRGTKRNVDR
FKISPNKIKELRTGEFIIYRTAENVNLPPQKVYVRNALEWLQKSNSK

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(587-686)

Helicase HerA, central domain (DUF87)

(217-408)

  Protein structure


Source ID Structure
AlphaFold DB Q8VT41

  Reference


[1] Francia MV et al. (2004) A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531]
[2] Francia MV et al. (2002) Transfer origins in the conjugative Enterococcus faecalis plasmids pAD1 and pAM373: identification of the pAD1 nic site, a specific relaxase and a possible TraG-like protein. Mol Microbiol. 45(2):375-95. [PMID:12123451]


Host bacterium

ID   200 GenBank   AH011360
Plasmid name   pAD1 Incompatibility group   -
Plasmid size   23020 bp Coordinate of oriT [Strand]   _
Host baterium   Enterococcus faecalis

Cargo genes

Drug resistance gene   _
Virulence gene   cylR2, cylR1
Metal resistance gene   _
Degradation gene   _
Symbiosis gene   -
Anti-CRISPR   AcrIIA9