Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100202
Name   oriT_pCI528 in_silico
Organism   Lactococcus lactis subsp. cremoris UC503
Sequence Completeness      intact
NCBI accession of oriT (coordinates [strand])   L08601 ( , 171 nt)
oriT length   171 nt
IRs (inverted repeats)      IR1: 10..19, 24..33  (CCCATTTTAT..ATAAAATGGG)
  IR2: 79..91, 95..107  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
  IR3: 113..127, 140..154  (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site      162..163
Conserved sequence flanking the
  nic site    		  CTTG|CA
Note   _

  oriT sequence  


Download         Length: 171 nt

>oriT_pCI528
GATCTTTTGCCCATTTTATTTTTATAAAATGGGCAGGTGGCGTTTGTGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGTTTTGTATTACAATGTGATAGCTTGCAGTATTTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Emond E et al. (2001) Molecular characterization of a theta replication plasmid and its use for development of a two-component food-grade cloning system for Lactococcus lactis. Appl Environ Microbiol. 67(4):1700-9. [PMID:11282624]


Relaxase

ID   195 GenBank   AAB28188
Name   Orf2_pCI528 insolico UniProt ID   Q79DL9
Length   410 a.a. PDB ID   
Note   relaxase

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47655.35 Da        Isoelectric Point: 8.1230

>AAB28188.1 mobilization protein (plasmid) [Lactococcus lactis]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDVSVSSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELEKR
TKQNEFSAVEQREPAITPLERDTQQRESEIVSLEQAIEPRKSEALKRESKINRFIDTIKQLAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure


Source ID Structure
AlphaFold DB Q79DL9


Host bacterium

ID   195 GenBank   L08601
Plasmid name   pCI528 Incompatibility group   _
Plasmid size   2006 bp Coordinate of oriT [Strand]   _
Host baterium   Lactococcus lactis subsp. cremoris UC503

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -