Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100201
• Name: oriT_pDR1-1
• Organism: Lactococcus lactis subsp. lactis bv. diacetylactis
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): NC_004164 (2..336 [+], 335 nt)
• oriT length: 335 nt
• IRs (inverted repeats): IR1: 10..19, 24..33 (CCCATTTTAT..ATAAAATGGG) IR2: 79..91, 95..107 (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: 113..127, 140..154 (CACATTGTAATACAA..TTGTATTACAATGTG)
• Location of nic site: 162..163
• Conserved sequence flanking the nic site: CTTG|CA
• Note: _

oriT sequence

• Length: 335 nt

>oriT_pDR1-1
GATCTTTTGCCCATTTTATTTTTATAAAATGGGCAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAAGTTCTTGATGGATCAGAAAAATGTATCACAAATTTAAACAAAG

oriT secondary structure

Predicted by RNAfold.

Reference

[1] Strahinic I et al. (2009) Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. J Appl Microbiol. 106(1):78-88. [PMID:19040703]

Host bacterium

• ID: 194
• GenBank: NC_004164
• Plasmid name: pDR1-1
• Incompatibility group: _
• Plasmid size: 7412 bp
• Coordinate of oriT [Strand]: 2..336 [+]
• Host baterium: Lactococcus lactis subsp. lactis bv. diacetylactis

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -