Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100200
• Name: oriT_pL2
• Organism: Lactococcus lactis subsp. lactis
• Sequence Completeness: intact
• NCBI accession of oriT (coordinates [strand]): DQ917780 (4772..5234 [+], 463 nt)
• oriT length: 463 nt
• IRs (inverted repeats): IR1: 138..147, 152..161 (CCCATTTTAT..ATAAAATGGG) IR2: 207..219, 223..235 (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: 241..255, 268..282 (CACATTGTAATACAA..TTGTATTACAATGTG)
• Location of nic site: 290..291
• Conserved sequence flanking the nic site: CTTG|CA
• Note: _

oriT sequence

• Length: 463 nt

>oriT_pL2
TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACAAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAAGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATACTTATTACAAAATAAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGTAAGTTATTGATGGATCAGAAAAATGTATCACAAATTGAAACAAAG

oriT secondary structure

Predicted by RNAfold.

Reference

[1] Chang SM et al. (2007) DNA sequence analysis of a cryptic plasmid pL2 from Lactococcus lactis subsp. lactis. Biotechnol Lett. 29(10):1519-27. [PMID:17563858]

Host bacterium

• ID: 193
• GenBank: DQ917780
• Plasmid name: pL2
• Incompatibility group: _
• Plasmid size: 5299 bp
• Coordinate of oriT [Strand]: 4772..5234 [+]
• Host baterium: Lactococcus lactis subsp. lactis

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -