Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100194
• Name: oriT_pTB19
• Organism: Bacillus stearothermophilus
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): M63891 (9869..9909 [+], 41 nt)
• oriT length: 41 nt
• IRs (inverted repeats): 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: TAGTGTG|CTAT
• Note: _

oriT sequence

• Length: 41 nt

>oriT_pTB19
CACTTTATGAATATAAAGTATAGTGTGCTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Reference

[1] Grohmann E et al. (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]

Relaxase

Mob_pTB19

• ID: 187
• GenBank: AAA98305
• Name: Mob_pTB19
• UniProt ID: Q03987
• Length: 415 a.a.
• Note: relaxase

Relaxase protein sequence

• Length: 415 a.a.
• Molecular weight: 48760.50 Da
• Isoelectric Point: 10.1984

>AAA98305.1 mobilizable protein (plasmid) [Geobacillus stearothermophilus]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDLLESELKNK
KSELAILSEEVSGEFKIPVKREKKSVEVPTGKRNLLGIEQKKTVMKSTGNVILKDEVFQDLKQKVKAGAV
LQTRVDQLLNTDYAKENQSLKKEVKELRSTNKSLSEENGRLKSTVEHLTNEIESLYVLTKDFLKARTNDL
ESFKELFGVFVGKVKEKAPRGLFVRQHERSEEKKNTFSLQDVLQRDRELREQRKAKRKKSHDLER

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: Q03987

Host bacterium

• ID: 187
• GenBank: M63891
• Plasmid name: pTB19
• Incompatibility group: -
• Plasmid size: 11887 bp
• Coordinate of oriT [Strand]: 9869..9909 [+]
• Host baterium: Bacillus stearothermophilus

Cargo genes

• Drug resistance gene: resistance to tetracycline, kanamycin, bleomycin
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -