Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100193
• Name: oriT_pIP823
• Organism: Listeria monocytogenes
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): U40997 ( , 41 nt)
• oriT length: 41 nt
• IRs (inverted repeats): 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: TAGTGTG|CTAT
• Note: _

oriT sequence

• Length: 41 nt

>oriT_pIP823
CACTTTATGAATATAAAGTATAGTGTGCTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Reference

[1] Grohmann E et al. (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]

Relaxase

Mob_pIP823

• ID: 186
• GenBank: AAA93296
• Name: Mob_pIP823
• UniProt ID: Q48764
• Length: 415 a.a.
• Note: relaxase

Relaxase protein sequence

• Length: 415 a.a.
• Molecular weight: 48841.75 Da
• Isoelectric Point: 10.0569

>AAA93296.2 recombination protein (plasmid) [Listeria monocytogenes]
MSFAVVRMQKMKSHDLKGIQFHNQRERESKTNPDIDKTRSHENYDLVNDEPIDYNERVKEIIESQKVGTR
KTRKDAVLVNELLVTSDRYFFERLDPDEQKRFFEESYKLFADRYGKQNIAYATVHMDEKTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMQKLGFDLQRGEKGSDREHIEMSKFKKQTLEKEIDLLENELKNK
KSELAILSEEVSGEFKIPVKREKKSVEVPTGKRNLLGIEQKKTVMKSTGNVILKDEVFQDLKKKVKAGAV
LQTRVDQLLNTDFAKENQSLKKEVKELRSTNKSLSEENSRLKSTVEHLTNEIESLYVLTKDFLKARTSDL
ESFKELFGVFVGKVKEKAPRGLFVRQHERSEEKKNTFSLQDVLQRDRELREQRKAKRKKSHDLER

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: Q48764

Host bacterium

• ID: 186
• GenBank: U40997
• Plasmid name: pIP823
• Incompatibility group: -
• Plasmid size: 3712 bp
• Coordinate of oriT [Strand]: _
• Host baterium: Listeria monocytogenes

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -