Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100192
• Name: oriT_pUB110
• Organism: Staphylococcus aureus
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): NC_001384 (1144..1181 [-], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: TAGTGTG|TTAT
• Note: _

oriT sequence

• Length: 38 nt

>oriT_pUB110
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Reference

[1] Grohmann E et al. (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]

Relaxase

Mob_pUB110

• ID: 185
• GenBank: NP_040431
• Name: Mob_pUB110
• UniProt ID: Q9KJC8
• Length: 420 a.a.
• Note: relaxase

Relaxase protein sequence

• Length: 420 a.a.
• Molecular weight: 49660.33 Da
• Isoelectric Point: 9.9693

>NP_040431.1 beta protein (plasmid) [Staphylococcus aureus]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: Q9KJC8

Host bacterium

• ID: 185
• GenBank: NC_001384
• Plasmid name: pUB110
• Incompatibility group: -
• Plasmid size: 4548 bp
• Coordinate of oriT [Strand]: 1144..1181 [-]
• Host baterium: Staphylococcus aureus

Cargo genes

• Drug resistance gene: aadD
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -