Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100191
Name   oriT_pTB53 in_silico
Organism   Bacillus sp.
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   D14852 ( , 38 nt)
oriT length   38 nt
IRs (inverted repeats)      2..8, 13..19  (ACTTTAT..ATAAAGT)
Location of nic site      27..28
Conserved sequence flanking the
  nic site    		  TAGTGTG|TTAT
Note   _

  oriT sequence  


Download         Length: 38 nt

>oriT_pTB53
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Grohmann E et al. (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]


Relaxase

ID   184 GenBank   BAA03580
Name   Pre_pTB53 insolico UniProt ID   Q52333
Length   414 a.a. PDB ID   
Note   relaxase

  Relaxase protein sequence


Download         Length: 414 a.a.        Molecular weight: 48699.55 Da        Isoelectric Point: 10.2722

>BAA03580.1 plasmid recombination enzyme (plasmid) [Bacillus sp.]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPVHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDLLESELKNK
KSELAILSEEVSGEFKIPVKREKKSVEVPTGKRNLLGIEQKKTVMKSTGNVILKDEVFQDLKQKVKAGAV
LQTRVDQLLNTDYAKENQSLKKEVKELRSTNKSLSEENGRLKSTVEHLTNEIESLYVLTKDFLKVRTNDL
ERFKELFGVFVGKVKEKAPRGLFVRHERSEEKKNTFSLQDVLQRDRELREQRKAKRKKSHDLER

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(1-193)


  Protein structure


Source ID Structure
AlphaFold DB Q52333


Host bacterium

ID   184 GenBank   D14852
Plasmid name   pTB53 Incompatibility group   -
Plasmid size   2083 bp Coordinate of oriT [Strand]   _
Host baterium   Bacillus sp.

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -