Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100190
• Name: oriT_pIP1714
• Organism: Staphylococcus cohnii
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): AF015628 (2356..2393 [+], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: TAGTGTG|TTAT
• Note: _

oriT sequence

• Length: 38 nt

>oriT_pIP1714
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Reference

[1] Grohmann E et al. (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]

Relaxase

Mob/Pre_pIP1714

• ID: 183
• GenBank: AAC61672
• Name: Mob/Pre_pIP1714
• UniProt ID: O87277
• Length: 420 a.a.
• Note: relaxase

Relaxase protein sequence

• Length: 420 a.a.
• Molecular weight: 49759.46 Da
• Isoelectric Point: 10.0119

>AAC61672.1 recombination/mobilization protein (plasmid) [Staphylococcus cohnii]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDRLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: O87277

Host bacterium

• ID: 183
• GenBank: AF015628
• Plasmid name: pIP1714
• Incompatibility group: -
• Plasmid size: 4978 bp
• Coordinate of oriT [Strand]: 2356..2393 [+]
• Host baterium: Staphylococcus cohnii

Cargo genes

• Drug resistance gene: resistance to streptogramins A and B
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -