Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   100188
Name   oriT_pGR71 in_silico
Organism   Bacillus subtilis (plasmid pGR71) K1 delta
Sequence Completeness      core
NCBI accession of oriT (coordinates [strand])   X15503 ( , 38 nt)
oriT length   38 nt
IRs (inverted repeats)      2..8, 13..19  (ACTTTAT..ATAAAGT)
Location of nic site      27..28
Conserved sequence flanking the
  nic site    		  TAGTGTG|TTAT
Note   _

  oriT sequence  


Download         Length: 38 nt

>oriT_pGR71
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Grohmann E et al. (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]


Relaxase

ID   181 GenBank   CAA33525
Name   Mob_pGR71 insolico UniProt ID   Q45626
Length   313 a.a. PDB ID   
Note   relaxase

  Relaxase protein sequence


Download         Length: 313 a.a.        Molecular weight: 37024.09 Da        Isoelectric Point: 10.0219

>CAA33525.1 unnamed protein product (plasmid) [Bacillus subtilis]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSFVSFDEIFRS

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(1-193)


  Protein structure


Source ID Structure
AlphaFold DB Q45626


Host bacterium

ID   181 GenBank   X15503
Plasmid name   pGR71 Incompatibility group   -
Plasmid size   1069 bp Coordinate of oriT [Strand]   _
Host baterium   Bacillus subtilis (plasmid pGR71) K1 delta

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -