Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 100186
• Name: oriT_pBC16
• Organism: Bacillus cereus
• Sequence Completeness: core
• NCBI accession of oriT (coordinates [strand]): NC_001705 (1144..1181 [-], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: TAGTGTG|TTAT
• Note: _

oriT sequence

• Length: 38 nt

>oriT_pBC16
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Reference

[1] Grohmann E et al. (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]

Relaxase

Mob_pBC16

• ID: 179
• GenBank: NP_043522
• Name: Mob_pBC16
• UniProt ID: Q51989
• Length: 441 a.a.
• Note: relaxase

Relaxase protein sequence

• Length: 441 a.a.
• Molecular weight: 51975.98 Da
• Isoelectric Point: 9.9596

>NP_043522.1 Mob (plasmid) [Bacillus cereus]
MVAGKSENASHLCHLKRSDLHMSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKND
KNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNI
AYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETA
KFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKP
TKNVVISERDYKNLVTAARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNE
LLEENKSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNE
FELTHNREVKKERSRDQGMSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (22-214)

Protein structure

• AlphaFold DB: Q51989

Host bacterium

• ID: 179
• GenBank: NC_001705
• Plasmid name: pBC16
• Incompatibility group: -
• Plasmid size: 4630 bp
• Coordinate of oriT [Strand]: 1144..1181 [-]
• Host baterium: Bacillus cereus

Cargo genes

• Drug resistance gene: tet(L)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -